To increase the fermentation yield and enhance the adaptability of a glutathione-producing engineering strain,optimize the growth cycle of the Saccharomyces cerevisiae engineering strain GSH21-1097,and inspect the stability of the strain.By examining the growth of single colonies,slopes,and shaking bottles of the strain at different time periods,the method by strain appearance,and the shake bottle detection of glutathione mass concentration,the optimal growth conditions for this strain have been determined.The suitable size for single colony cultivation for about 56 hours has a high glutathione content,which was 24.1％higher than the initial screening potency of GSH21-1097 engineering strains;after 24 hours of cultivation at 160 r/min and 28 ℃ in a shaking flask,cultivate a slope for 96 hours,and the yield of glutathione can reach 0.55 g/L;the optimal time for controlling the seeds inserted into the fermentation bottle was 18-20 hours;the glutathione mass concentration reached its peak after 33-35 hours of fermentation in a shaking flask.The optimized growth conditions of the strain were used to investigate the genetic stability of the genetic strain.Within ten generations,the genetic performance remained stable,exhibiting only a slight reduction in shake flask potency and retaining its long-term storage capacity.This study optimized the appropriate conditions for the growth of the Saccharomyces cerevisiae engineering strain GSH21-1097,which improved its ability to produce glutathione and increased the genetic stability of the strains.
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