As a natural organic acid,lactic acid is widely used in medicine,food,agriculture,industry and,biomaterials.To meet the market demand for lactic acid,research on production cost reduction,production condition optimization,high-yield lactic acid strain screening and their application in industrial production of lactic acid has become a hotpoint.The high-yielding lactic acid strain LBZ2 was obtained through laboratory mutagenesis breeding,and this strain was used to perform immobilized lactic acid fermentation.A single factor experiment was conducted to optimize the conditions for immobilized lactic acid production using the response surface method,and the acid production capacity of LBZ2 liquid fermentation and immobilized embedding was compared.The results showed that the acid yield of lactic acid strain LBZ2 was high after immobilized fermentation,and the optimal acid production conditions were 2.3％for K-type carrageenan,15.3％for LBZ2 inoculation,3.0％for KCl in solidifying solution,and 48 h for fermentation time,under which the lactic acid yield was 84.66 g/L and the volume yield was 1.763 g/(L·h).
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