1H-qNMR for the quantification of etomidate in suspicious samples
A 1H-quantitative nuclear magnetic resonance spectrometry(1H-qNMR)method was established for the quantification of etomidate in suspicious solid and electronic cigarette oil samples.According to the solubility and stability of etomidate in different deuterated solvents,and the spectra comparison of seized samples and reference materials of common adulterants,dimethylsulfoxid-d6 was selected as the solvent,and dimethyl terephthalate was selected as the internal standard.The signal at δH 8.09 ppm for internal standard dimethyl terephthalate and that at δH of 8.30 and 6.25 ppm for etomidate were selected as quantitative peaks.The limit of quantitation of this method was 0.05%,the relative standard deviations(RSDs)of intra-day and inter-day were 0.05%and 0.2%,respectively.The established 1H-qNMR method was applied for the quantification of etomidate in four solid samples and four electronic cigarette oil seized samples.The contents of etomidate in 8 samples were 6.23%-99.70%,which were basically consistent with the results of high-performance liquid chromatography.This study provides new insights into the applying of 1H-qNMR for the quantification of etomidate in seized psychotropic substance samples.
1H-quantitative nuclear magnetic resonance spectroscopy(1H-qNMR)etomidatequantitative analysispsychotropic substance
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