Fluorescence-activated cell sorting based on ultrasonic acoustic fluid tunneling
This paper develops a complete fluorescence-activated cell sorting(FACS)system based on acoustofluidic technology.This system uses the acoustofluidic tunnel in the microfluidic channel generated by a solidly mounted film bulk acoustic wave resonator(SMR)and combines it with the fluorescence recognition activation program to achieve high-precision capture and sorting of individual target particle and cell,having the function of detecting and sorting target particles.The stability of the system was verified by separating fluorescent microspheres from a mixture of non-fluorescent and fluorescent polystyrene microspheres.The purity could reach 95%at a throughput of 150 cells/s.For cell samples,live HeLa cells were isolated from live and dead HeLa cells with a purity higher than 90%,proving the high resolution of this system in sorting live and dead cells.And the survival rates of cells before and after sorting were higher than 95%,proving the good biocompatibility of the system.
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