Determination of nonivamide in guinea pig plasma by high performance liquid chromatography-tandem mass spectrometry
A method was established for the determination of trace nonivamide(NOV)in guinea pig plasma by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Capsaicin(CAP)was chosen as the internal standard.Protein was precipitated with acetonitrile in the plasma samples,then the samples were centrifuged at high speed,dried and concentrated under vacuum condition,and finally prepared by mobile phase reconstitution.The Agilent EclipsePlus C18 column(2.1 mm×150 mm,1.8 μm)was used.The mobile phases consisted of acetonitrile and water containing 0.1%formic acid.The electrospray ionization(ESI)source and multiple reaction monitoring(MRM)mode were used for the detection.Using gradient elution program,the peak time of both NOV and the internal standard(CAP)was 5.31 min.m/z 294.2→137.1 was selected as the quantification ion of NOV,and m/z 306.2→137.1 as the internal standard quantification ion.The matrix effects of NOV in guinea pig plasma were 98.6%-104.0%,the recoveries were 110.4%-117.4%,and the inter-day relative standard deviations were 0.2%-2.6%.The linear relationship was good in the concentration range of 0.05-50 ng/mL,with the correlation coefficient(R2)of 0.9998.The limit of quantification was 0.05 ng/mL.This method can meet the detection needs of trace NOV in plasma and can be used to carry out the quantitative analysis and related mechanism research of NOV after inhalation exposure as a novel riot control agent.
high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)guinea pig plasmanonivamide(NOV)capsaicin(CAP)
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