Fluorescence detection of artemisinin based on papain as a catalyst
In this study,a fluorescence method for artemisinin detection was established based on the principle that the peroxisome bridges can be broken by papain,releasing large amounts of reactive free radicals and oxidizing O-Phenylenediamine(OPD)to the fluorescent product 2,3-diaminophenazine(DAP),which can generate fluorescence emission at 550 nm to be used for the detection of artemisinin.Under the optimum conditions,the linear range of artemisinin was 50-2000 μmol/L,and the limit of detection was 0.8 μmol/L.The interference experiment showed that this method had good selectivity for artemisinin.This method has been successfully applied to determine the contents of artemisinin in artemisinin piperaquine tablets,with a relative error of less than 6.28%.The satisfactory accuracy confirms its great potential for practical sample analysis.
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