In this study,a fluorescence method for artemisinin detection was established based on the principle that the peroxisome bridges can be broken by papain,releasing large amounts of reactive free radicals and oxidizing O-Phenylenediamine(OPD)to the fluorescent product 2,3-diaminophenazine(DAP),which can generate fluorescence emission at 550 nm to be used for the detection of artemisinin.Under the optimum conditions,the linear range of artemisinin was 50-2000 μmol/L,and the limit of detection was 0.8 μmol/L.The interference experiment showed that this method had good selectivity for artemisinin.This method has been successfully applied to determine the contents of artemisinin in artemisinin piperaquine tablets,with a relative error of less than 6.28%.The satisfactory accuracy confirms its great potential for practical sample analysis.
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