以木瓜蛋白酶为催化剂的青蒿素的荧光检测
Fluorescence detection of artemisinin based on papain as a catalyst
刘晓霞 1侯文雅 1郭晓迪 1赵晋忠 1赵武勇2
作者信息
- 1. 山西农业大学基础部,晋中 030801
- 2. 山西白求恩医院康复科,太原 030032
- 折叠
摘要
本研究建立了一种药物中青蒿素(ART)的荧光检测法.青蒿素的过氧桥结构可以被具有过氧化物活性的木瓜蛋白酶催化打破,释放出大量的活性自由基并将邻苯二胺(OPD)氧化为荧光产物2,3-二氨基吩嗪(DAP),此荧光产物可在550 nm产生荧光发射,可用于测定青蒿素的含量.在最佳条件下,青蒿素的线性范围是50～2000 µmol/L,检出限为0.8 µmol/L,干扰实验表明该方法对青蒿素具有良好的选择性.该方法已成功用于检测青蒿素哌喹片中青蒿素的含量,相对误差小于6.28%.该方法在实际样品中ART的检测方面具有一定的应用前景.
Abstract
In this study,a fluorescence method for artemisinin detection was established based on the principle that the peroxisome bridges can be broken by papain,releasing large amounts of reactive free radicals and oxidizing O-Phenylenediamine(OPD)to the fluorescent product 2,3-diaminophenazine(DAP),which can generate fluorescence emission at 550 nm to be used for the detection of artemisinin.Under the optimum conditions,the linear range of artemisinin was 50-2000 μmol/L,and the limit of detection was 0.8 μmol/L.The interference experiment showed that this method had good selectivity for artemisinin.This method has been successfully applied to determine the contents of artemisinin in artemisinin piperaquine tablets,with a relative error of less than 6.28%.The satisfactory accuracy confirms its great potential for practical sample analysis.
关键词
木瓜蛋白酶/过氧化物活性/青蒿素/荧光法Key words
papain/peroxide activity/artemisinin/fluorescence method引用本文复制引用
出版年
2025
NETL
NSTL
万方数据