Application of digital PCR technology in the field of entry and exit port biosecurity assurance
As the third generation PCR detection technology,digital PCR can absolutely quantify the initial concentration of nucleic acid in samples without calibrators and standard curves,which has the characteristics of high sensitivity,high specificity and high accuracy.It has been widely used in the diagnosis of genetic diseases,cancer,infectious diseases,and the study of copy number variation,mutation detection,transgene detection and other aspects.In this paper,the development,principle and data analysis methods of digital PCR technology are introduced,the research progress and application prospect of digital PCR technology in the field of customs bio-security are introduced and discussed.
Digital PCRHigh SensitivityAbsolutely quantificationCustoms bio-security
NETL
NSTL
万方数据
维普
