This study used commercially available bitter gourd as the material and extracted polysaccharides from bitter gourd powder using pectinase.The effects of enzymatic hydrolysis temperature,hydrolysis time,pectinase dosage,and pH on the extraction rate of bitter gourd polysaccharides were investigated.The extraction rate of bitter gourd polysaccharides was de-termined through single factor experiments,and the extraction process of bitter gourd polysaccharides was optimized using re-sponse surface method.The antioxidant capacity of bitter gourd polysaccharides was measured using DPPH free radical and ABTS free radical scavenging ability as indicators,and the antibacterial activity of bitter gourd polysaccharides was determined by the size of the inhibition zone.It was found that the extraction rate of bitter gourd polysaccharides was the highest at 14.74％in an environment with a temperature of 54 ℃,an enzymatic hydrolysis time of 86 minutes,a pectinase addition of 0.05 g,and a pH of 4.86.Through antioxidant tests,it was found that bitter gourd polysaccharides have a good ability to scavenge DPPH and ABTS free radicals.Through antibacterial tests,it was found that bitter gourd polysaccharides can effectively inhibit the prolifer-ation of Escherichia coli and Staphylococcus aureus.
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