三种超低温冷冻保存香椿种子的研究初探
Preliminary study on three types of ultra low temperature preservation of Toona sinensis seeds
陈清怡 1刘雅琪 1鲍芙蓉 1余世玉 1苏芊妮 1屈长青2
作者信息
- 1. 阜阳师范大学 生物与食品工程学院,安徽 阜阳 236037
- 2. 阜阳师范大学 生物与食品工程学院,安徽 阜阳 236037;抗衰老中草药安徽省工程技术研究中心,安徽 阜阳 236037
- 折叠
摘要
香椿是具有极高应用价值的优良资源植物.为了能够稳定保存优良香椿种质资源,本研究采用 3 种超低温保存方法处理香椿种子,通过发芽率和生理生化指标检测,对比了三种香椿种子低温保存方法的优劣.结果表明,经过冷冻后,香椿种子的超氧化物歧化酶活性和过氧化氢酶活性均升高,过氧化物酶活性和MDA含量均降低.但玻璃化冷冻对种子的发芽率和可溶性蛋白含量与对照组相比无显著差异,因此玻璃化冷冻法更适合香椿种子的超低温冷冻保存.
Abstract
Toona sinensis is an excellent resource plant with extremely high application value.In order to stabilize the pres-ervation of excellent Toona sinensis germplasm resources,this study used three ultra-low temperature preservation methods to treat Toona sinensis seeds.The germination rate and physiological and biochemical indicators were tested to compare the advan-tages and disadvantages of the three low-temperature preservation methods for Toona sinensis seeds.The results showed that af-ter freezing,the superoxide dismutase activity and catalase activity of Toona sinensis seeds increased,while the peroxidase activ-ity and MDA content decreased.However,there was no significant difference in germination rate and soluble protein content be-tween the vitrification freezing group and the control group,so vitrification freezing method is more suitable for ultra-low tem-perature freezing preservation of Toona sinensis seeds.
关键词
香椿种子/超低温保存/发芽率/生理生化特性Key words
Toona sinensizs seeds/ultra-low temperature preservation/germination rate/physiological and biochemical in-dicators引用本文复制引用
基金项目
安徽省科技重大专项(202003a06020020)
出版年
2024
NETL
NSTL
万方数据