Objective To investigate compare the difference of local early delayed type hypersensitivity induced by two kinds of non-absorbable silk suture in intraperitoneal and extraperitoneal abdominal wall of mice.Method Selected 7 Balb/C mice (10-12 weeks),three vertical and parallel incisions with 5 mm length from left to right were created in abdominal wall in each mice,The deep wall of abdominal wall was sutured with 4-0 MERSILK® non-absorbable suture with needle mousse line and knotted in the extraperitoneal space (MERSILK® extraperitoneal knotting group),knotted in the wall peritoneum (MERSILK® intraperitoneal knotting group) and the wall peritoneum was knotted with 4-0 Proline suture through the abdominal wall incision (Proline suture intraperitoneal knotting group).At 7 days after operation,the abdominal wall specimens of the three groups were taken.Result The results of mmunohistochemistry staining and quantitative evaluation showed that the expression of antigen peptide-major histocompatibility complex (MHC) class Ⅰ/Ⅱ molecules complex,CD4+,interferon-γ (IFN-γ) and lymphotoxin-β (LT-β) in the Proline suture intraperitoneal knotting group were less than those in the MERSILK® extraperitoneal knotting group and the MERSILK® intraperitoneal knotting group,However,there was no significant difference between the MERSILK® extraperitoneal knotting group and MERSILK® intraperitoneal knotting group.Masson staining showed that there were flaky fibrous collagen cords around the knot in the Proline suture intraperitoneal knot-tying group,and the collagen fibers were arranged relatively uniformly.In the MERSILK® extraperitoneal knotting group and MERSILK® intraperitoneal knotting group only scattered fibrous collagen cords were observed and arranged loosely.Conclusion Proline® suture knot induces less early delayed hypersensitivity around the abdominal wall knot than MERSILK® silk suture.When the inner ring is closed under laparoscopic monitoring,the use of Proline suture intraperitoneal purse-string suture knot is less hypersensitive than the use of MERSILK® silk sutured and intraperitoneal purse-string suture knot,which is relatively ideal.
关键词
慕斯线/Proline线/腹腔内/腹膜外/线结/超敏反应
Key words
MERSILK® silk suture/Proline® suture/Intracorporeal/Extracorporeal/Knot/Delayed type hypersensitivity
维普
万方数据