Histological observation of goblet cell and mucoprotein 2 expression in mucosa of septum arisen from type Ⅰ intestinal atresia
Objective To observe the expression of goblet cell and mucoprotein 2(MUC2)in mucosa of septum arisen from type Ⅰ intestinal atresia.Method Totally 9 septa located in intestinal wall with distance less than 15 cm from Treitz ligament(septum group)were collected.The age of the patients'operation were 1-3 days after birth.The normal intestinal wall tissues were harvested and set as the control during resection and anastomosis in the same patient(control group),followed by serial tissue sectioning and following hematoxylin-eosin(HE)staining,periodic acid-Scheff(PAS)staining,as well immunohistochemistry staining for histological observation.The semi-quantitative calculation was conducted for statistical comparison.Statistical method performed by independent sample t test.Result HE staining showed mucous of septum arisen from type Ⅰ intestinal atresia presented relatively normal histological structure with less villi and malalignment.Submucosa muscular layer was irregular and proliferating collagen in subcutaneous layer.The residual goblet cells were less in septum group(36.6±2.6)compared with control group(72.5±3.7),with the difference was significant(t=11.235,P=0.001).PAS staining indicated that glycoprotein in goblet cell was denser and immunohistochemistry staining showed MUC2 was less-expressed in septum group(0.085 0±0.005 4)compared with control group(0.115 6±0.014 4),with the difference was significant(t=18.652,P=0.014).Conclusion Compared with the normal intestinal wall,the septum arisen from type Ⅰ intestinal atresia has disorganized microscopic structure and relatively less villi,and present imperfect developmental feature,suggesting weaker defensive function induced by goblet cell in contrast to the normal intestinal wall.
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