Synthesis of Glycerol Ferulate Catalysed by NaBH4@AuNPs-CRL Hybridases Prepared by in situ Reduction of Sodium Borohydride
In this work,a method based on the in situ reduction of sodium borohydride for the preparation of gold nanobiohybrids for the synthesis of glycerol ferulate was established,which resulted in better activity,stability and structural rigidity of the hybridases.The optimum conditions for the preparation of the NaBH4@AuNPs-CRL hybridases were obtained by a one-way experiment,and the changes of the lipase before and after hybridisation were explored using fluorescence spectroscopy,infrared spectroscopy,X-ray electron spectroscopy and transmission electron microscopy.It has been shown that the NaBH4@AuNPs-CRL hybridases have a reduction of a-helix and an increase of β-folding in their secondary structure.The specific activity of the NaBH4@AuNPs-CRL hybridases was 4.91±0.12 U·mg-1,which was 66.44%higher compared to CRL.The conversion of the glycerol ferulate synthesis catalysed by the NaBH4@AuNPs-CRL hybridases under the optimum conditions was 98.39%±3.65%,and the stability experiments carried out in batch mode showed that both the activity and the stability had been improved.