首页|AIE型荧光探针在四环素类抗生素检测上的应用

AIE型荧光探针在四环素类抗生素检测上的应用

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本文以具有AIE效应的典型代表TPB作为荧光探针，利用UV-Vis和FT-IR等技术表征了 TPB，以紫外光谱和荧光光谱分析法探究了 TPB的光学性质，实验结果进一步证明了 AIE效应。通过对不同种类9种抗生素对TPB的淬灭实验结果对比，发现四环素类抗生素淬灭TPB的程度最大，而其他种类抗生素效果不明显，实现了四环素类抗生素的特异性识别，并以四环素类抗生素中的土霉素为例，探究抗生素溶液浓度对荧光淬灭效果的定量关系，并计算出了检出限。通过对荧光淬灭机理探究发现，盐酸四环素的紫外吸收光谱和TPB的荧光激发光谱具有较高的重叠性，而磺胺二甲氧嘧啶等其他种类抗生素的紫外吸收光谱和TPB的荧光激发光谱的重叠性较小，满足荧光内滤效应。TPB荧光探针性质稳定，价格低廉，抗干扰能力较强，本方法在四环素的实际检测中更具有应用价值。
Applying an AIE type fluorescent probe in the detection of tetracycline antibiotics
The typical representative TPB(tetraphenyl-1,3-butadiene)molecule with AIE effect was used as a fluorescent probe,and the specific recognition of tetracycline antibiotics was achieved through the comparison of the experimental results of the quenching of TPB by nine antibiotics of different types,which indicated that tetracycline antibiotics were found to quench TPB to the greatest extent,while the quenching of other antibiotics(e.q.chloramphenicol,aminoglycoside,sulfonamides,and quinolone)was not obvious.Then the quantitative relationship between the concentration of antibiotic solution and the effect of fluorescence quench was investigated by taking oxytetracycline among tetracycline antibiotics as an example,and the detection limit was calculated.Finally,it was found that the UV absorption spectra of tetracycline hydrochloride and the fluorescence excitation spectra of TPB had high overlap,while the o-verlap between the UV absorption spectra of other antibiotics,such as sulfadimethoxine,and the fluores-cence laser spectrum of TPB was low,which fulfilled the fluorescence internal filtering effect.The fluores-cence probe has stable properties,low cost and strong anti-interference ability,which makes the method more valuable in the practical detection of tetracycline.This study indicates great potent in the detection of antibiotics.

fluorescence detection technologytetraphenyl-1,3-butadienetetracycline antibiotics

胡博、郑露莹、王瑞、许堃、赵思思、于湛、陈庆阳、张航

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沈阳师范大学化学化工学院,辽宁沈阳 110034

荧光检测技术 四苯基-1,3-丁二烯 四环素类抗生素

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaInnovation Entrepreneurship Training Program for College Students of the School of Chemistry and Chemical Engineering at Shenyan

2210910522102106

2024

10.13563/j.cnki.jmolsci.2023.12.006

分子科学学报
中国化学会

分子科学学报

CSTPCD
影响因子：0.434
ISSN：1000-9035
年,卷(期)：2024.40(2)
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