Preparation and antiviral effects of polysaccharides from Panax japonicus
Water-soluble polysaccharides were prepared from Panax japonicus by hot water extraction and ethanol precipitation.The polysaccharides were further purified by ion exchange chromatography to obtain neutral and acidic polysaccharides.The neutral polysaccharide fraction mainly contained Glc(90.2％),which was a glucan fraction.The acidic polysaccharide fraction mainly contained GalA(43.6％),Gal(21.7％),and Ara(15.4％),with a degree of methyl-esterification of 20.3％,which was a pectic poly-saccharide.The acidic polysaccharide of Panax japonicus could effectively inhibit the replication of human seasonal influenza virus H1N1 and canine influenza virus H3N2 in MDCK cells and A549 cells and signifi-cantly reduce the virus titer in infected cells.It also effectively inhibited the number of infected cells of the SARS-CoV-2 South Africa strain and the Omicron strain.The acid polysaccharide of Panax japonicus showed good efficacy against influenza virus and COVID-19 infection,which could be used as a potential antiviral candidate drug molecule in the future.
Panax japonicus C.A.Meyerpolysaccharidesisolation and purificationanti-influenza virusinhibition of SARS-CoV-2
朱雪鹏、申蓓蕾、崔梁楠、孙琳、高玉伟、周义发
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东北师范大学生命科学学院,糖复合物教育部工程研究中心,吉林长春 130024
军事科学院军事医学研究院军事兽医研究所,吉林长春 130122
竹节参 多糖 分离纯化 抗流感病毒 抗新冠病毒
National Key Research and Development Program of China
NETL
NSTL
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