Polyoxometalate-based nanozyme with supramolecular structure exhibit laccase-mimicking activity for the detection of kanamycin
Kanamycin is a commonly used aminoglycoside anti-infective drug with good antimicrobial effects against most Enterobacteriaceae bacteria and is often used in animal husbandry and aquaculture.However,excessive amount of kanamycin can be a threat to human health.Therefore,it is crucial to develop a con-cise and rapid assay for kanamycin.Colorimetric assay based on nanozymes is considered as one of the ef-fective methods for rapid and efficient detection of pollutants.Compared with natural enzymes,nanozymes have the advantages of simple synthesis,controllable cost,high stability and high catalytic efficiency.Therefore,a polyoxometalates-based material,[H3PW12O40(BPE)2.5]·3H2O(PW12/BPE)(BPE=1,2-bis(4-pyridyl)ethane),was synthesized by hydrothermal method in this work.PW12/BPE exhibited satis-factory laccase-like activity as a nanozyme.Kinetic study showed that the laccase-mimicking performance of PW12/BPE is low in Km but high in Vmax.Colorimetric assay was carried out for the content of kanamy-cin based on the laccase-like performance of PW12/BPE.The results exhibited a good linear relationship between kanamycin concentration and absorbance,and the LOD was as low as 16.66 μmol·L-1.
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