具有类漆酶特性的超分子多金属氧酸盐基纳米酶在卡那霉素检测上的应用
Polyoxometalate-based nanozyme with supramolecular structure exhibit laccase-mimicking activity for the detection of kanamycin
鲁俊俊 1徐欣欣1
作者信息
- 1. 东北大学理学院,辽宁沈阳 110819
- 折叠
摘要
卡那霉素是一种常用的氨基糖苷类抗感染药物,对大多数肠杆菌科细菌具有良好的抗菌作用,常被用于畜牧业和水产养殖.而过量的卡那霉素会对人体健康造成威胁.因此开发一种简洁快速的卡那霉素的检测方法至关重要.基于纳米酶的比色检测法被认为是快速高效检测污染物的有效方法之一.与天然酶相比,纳米酶具有合成简单、成本可控、稳定性强及催化效率高等优点.因此,本文采用水热法合成了 一种多金属氧酸盐材料[H3PW12O40(BPE)2.5]·3H2O(PW12/BPE)(BPE=1,2-双(4-吡啶基)乙烷).PW12/BPE 作为纳米酶表现优异的漆酶模拟活性.动力学研究表明PW12/BPE的漆酶模拟性能具有较低的Km和较高的Vmax.基于PW12/BPE的类漆酶性能对卡那霉素的含量进行紫外-可见光谱比色检测.结果表明卡那霉素浓度与吸光度呈良好的线性关系,并且检测限低至16.66 μmol·L-1.
Abstract
Kanamycin is a commonly used aminoglycoside anti-infective drug with good antimicrobial effects against most Enterobacteriaceae bacteria and is often used in animal husbandry and aquaculture.However,excessive amount of kanamycin can be a threat to human health.Therefore,it is crucial to develop a con-cise and rapid assay for kanamycin.Colorimetric assay based on nanozymes is considered as one of the ef-fective methods for rapid and efficient detection of pollutants.Compared with natural enzymes,nanozymes have the advantages of simple synthesis,controllable cost,high stability and high catalytic efficiency.Therefore,a polyoxometalates-based material,[H3PW12O40(BPE)2.5]·3H2O(PW12/BPE)(BPE=1,2-bis(4-pyridyl)ethane),was synthesized by hydrothermal method in this work.PW12/BPE exhibited satis-factory laccase-like activity as a nanozyme.Kinetic study showed that the laccase-mimicking performance of PW12/BPE is low in Km but high in Vmax.Colorimetric assay was carried out for the content of kanamy-cin based on the laccase-like performance of PW12/BPE.The results exhibited a good linear relationship between kanamycin concentration and absorbance,and the LOD was as low as 16.66 μmol·L-1.
关键词
纳米酶/多金属氧酸盐/漆酶/卡那霉素/比色检测Key words
nanozyme/polyoxometalates/laccase/kanamycin/colorimetric assay引用本文复制引用
出版年
2024
NETL
NSTL
万方数据