首页|ORS1,an H2O2-Responsive NAC Transcription Factor,Controls Senescence in Arabidopsis thaliana

ORS1,an H2O2-Responsive NAC Transcription Factor,Controls Senescence in Arabidopsis thaliana

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We report here that ORS1,a previously uncharacterized member of the NAC transcription factor family,controls leaf senescence in Arabidopsis thaliana. Overexpression of ORS1 accelerates senescence in transgenic plants,whereas its inhibition delays it. Genes acting downstream of ORS1 were identified by global expression analysis using transgenic plants producing dexamethasone-inducible ORS1-GR fusion protein. Of the 42 up-regulated genes,30 (～70%) were previously shown to be up-regulated during age-dependent senescence. We also observed that 32 (～76%) of the ORS1-de-pendent genes were induced by long-term (4 d),but not short-term (6 h) salinity stress (150 mM NaCI). Furthermore,expression of 16 and 24 genes,respectively,was induced after 1 and 5 h of treatment with hydrogen peroxide (H2O2),a reactive oxygen species known to accumulate during salinity stress. ORS1 itself was found to be rapidly and strongly induced by H2O2 treatment in both leaves and roots. Using in vitro binding site selection,we determined the preferred binding motif of ORS1 and found it to be present in half of the ORS1 -dependent genes. ORS1 is a paralog of ORE1/ ANAC092/AtNAC2,a previously reported regulator of leaf senescence. Phylogenetic footprinting revealed evolutionary conservation of the ORS1 and ORE1 promoter sequences in different Brassicaceae species,indicating strong positive selection acting on both genes. We conclude that ORS1,similarly to ORE1,triggers expression of senescence-associated genes through a regulatory network that may involve cross-talk with salt- and H2O2-dependent signaling pathways.

NAC transcription factorleaf senescencegene expressiongene regulatory networkhydrogen peroxide

Salma Balazadeh、Miroslaw Kwasniewski、Camila Caldana、Mohammad Mehrnia、María Inés Zanor、Gang-Ping Xue、Bernd Mueller-Roeber

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University of Potsdam,Institute of Biochemistry and Biology,Karl-Liebknecht-Straβe 24-25,Haus 20,14476 Potsdam-Golm,Germany

Max-Planck Institute of Molecular Plant Physiology,Am Muhlenberg 1,14476 Potsdam-Golm,Germany

University of Silesia,Department of Genetics,Jagiellonska 28,40-032,Katowice,Poland

Present address: Institute de Biologia Molecular y Celular de Rosario (IBR-CONICET). Facultad de Ciencias Bioquimicas y Farmacéuticas (UNR) Suipacha 530,Rosario 52002LRK,Argentina

CSIRO Plant Industry,306 Carmody Road,St Lucia,QLD 4067,Australia

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Funding was provided by BMBF (GABI Program)DAADDeutsche Forschungsgemein-schaft (FOR 948-Nitrogen uptake,metabolism,and remobilization in leaves during plant senescence)

FKZ 0312276MNo.A/06/04209MU 1199/14-1

2011

10.1093/mp/ssq080

分子植物(英文版)
中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCDCSCDSCI
影响因子：0.659
ISSN：1674-2052
年,卷(期)：2011.4(2)
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