首页|H2A.Z Represses Gene Expression by Modulating Promoter Nucleosome Structure and Enhancer Histone Modifications in Arabidopsis
H2A.Z Represses Gene Expression by Modulating Promoter Nucleosome Structure and Enhancer Histone Modifications in Arabidopsis
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Deposition of the histone variant H2A.Z at gene bodies regulates transcription by modifying chromatin accessibility in plants.However,the role of H2A.Z enrichment at the promoter and enhancer regions is unclear,and how H2A.Z interacts with other mechanisms of chromatin modification to regulate gene expression remains obscure.Here,we mapped genome-wide H2A.Z,H3K4me3,H3K27me3,Pol Ⅱ,and nucleosome occupancy in Arabidopsis inflorescence.We showed that H2A.Z preferentially associated with H3K4me3 at promoters,while it was found with H3K27me3 at enhancers,and that H2A.Z deposition negatively correlated with gene expression.In addition,we demonstrated that H2A.Z represses gene expression by establishing low gene accessibility at +1 nucleosome and maintaining high gene accessibility at-1 nucleosome.We further showed that the high measures of gene responsiveness correlate with the H2A.Z-associated closed +1 nucleosome structure.Moreover,we found that H2A.Z represses enhancer activity by promoting H3K27me3 and preventing H3K4me3 histone modifications.This study provides a framework for future studies of H2A.Z functions and opens up new aspects for decoding the interplay between chromatin modification and histone variants in transcriptional control.
Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Center for Genomics and Biotechnology, College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China
Institute of Ocean Science, Minjiang University, Fuzhou 350108, China
This work was supported by NSFCThis work was supported by NSFCThis work was supported by NSFCThis work was supported by NSFCThis work was supported by NSFCNewton Advanced Fellowship to Y.Q.,Natural Science Foundation of Fujian Province to L.Zfunds from Fujian Innovative Center for Germplasm Resources
NETL
NSTL
万方数据
维普
