首页|Activation of Mitochondrial orf355 Gene Expression by a Nuclear-Encoded DREB Transcription Factor Causes Cytoplasmic Male Sterility in Maize
Activation of Mitochondrial orf355 Gene Expression by a Nuclear-Encoded DREB Transcription Factor Causes Cytoplasmic Male Sterility in Maize
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Coordination between mitochondria and the nucleus is crucial for fertility determination in plants with cytoplasmic male sterility (CMS).Using yeast one-hybrid screening,we identified a transcription factor,ZmDREB1.7,that is highly expressed in sterile microspores at the large vacuole stage and activates the expression of mitochondria-encoded CMS gene orf355.△pro,a weak allele of ZmDREB1.7 with the loss of a key unfolded protein response (UPR) motif in the promoter,partially restores male fertility of CMS-S maize.ZmDREB1.7 expression increases rapidly in response to antimycin A treatment,but this response is attenuated in the △pro allele.Furthermore,we found that expression of orf355 in mitochondria activates mitochondrial retrograde signaling,which in turn induces ZmDREB1.7 expression.Taken together,these findings demonstrate that positive-feedback transcriptional regulation between a nuclear regulator and a mitochondrial CMS gene determines male sterility in maize,providing new insights into nucleusmitochondria communication in plants.
maizecytoplasmic male sterilitytranscription factornucleus-mitochondria communication
State Key Laboratory of Plant Cell and Chromosome Engineering, Innovative Academy of Seed Design, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences,Beijing, 100097, China
University of Chinese Academy of Sciences, Beijing 100864, China
This work was supported by grants from the National Key Research and Development Programs of ChinaThis work was supported by grants from the National Key Research and Development Programs of China
NETL
NSTL
万方数据
维普
