首页|Multiomics analyses of two Leonurus species illuminate leonurine biosynthesis and its evolution

Multiomics analyses of two Leonurus species illuminate leonurine biosynthesis and its evolution

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The Lamiaceae family is renowned for its terpenoid-based medicinal components,but Leonurus,which has traditional medicinal uses,stands out for its alkaloid-rich composition.Leonurine,the principal active com-pound found in Leonurus,has demonstrated promising effects in reducing blood lipids and treating strokes.However,the biosynthetic pathway of leonurine remains largely unexplored.Here,we present the chromosome-level genome sequence assemblies of Leonurus japonicus,known for its high leonurine production,and Leonurus sibiricus,characterized by very limited leonurine production.By integrating ge-nomics,RNA sequencing,metabolomics,and enzyme activity assay data,we constructed the leonurine biosynthesis pathway and identified the arginine decarboxylase(ADC),uridine diphosphate glucosyltrans-ferase(UGT),and serine carboxypeptidase-like(SCPL)acyltransferase enzymes that catalyze key reac-tions in this pathway.Further analyses revealed that the UGT-SCPL gene cluster evolved by gene duplica-tion in the ancestor of Leonurus and neofunctionalization of SCPL in L.japonicus,which contributed to the accumulation of leonurine specifically in L.japonicus.Collectively,our comprehensive study illuminates leonurine biosynthesis and its evolution in Leonurus.

LamiaceaeLeonurus japonicusLeonurus sibiricusgenome sequencealkaloidsleonurinegene clusterbiosynthetic pathwayevolution

Peng Li、Meng-Xiao Yan、Pan Liu、Dan-Jie Yang、Ze-Kun He、Yun Gao、Yan Jiang、Yu Kong、Xin Zhong、Sheng Wu、Jun Yang、Hong-Xia Wang、Yan-Bo Huang、Le Wang、Xiao-Ya Chen、Yong-Hong Hu、Qing Zhao、Ping Xu

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Shanghai Key Laboratory of Plant Functional Genomics and Resources,Shanghai Chenshan Botanical Garden,Shanghai Chenshan Plant Science Research Center,Chinese Academy of Sciences,Shanghai,China

College of Life Sciences,Shanghai Normal University,Shanghai 200234,China

State Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai,China

Universityofthe Chinese Academy of Sciences,Beijing 100049,China

The Research Center of Chiral Drugs,Innovation Research Institute of Traditional Chinese Medicine,Shanghai University of Traditional Chinese Medicine,1200 Cailun Road,Shanghai 201203,China

College of Chemistry and Chemical Engineering,Shanghai University of Engineering Science,Shanghai 201620,China

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National Natural Science Foundation of ChinaChenshan Special Fund for the Shanghai Landscaping Administration Bureau ProgramMinistry of Science and Technology of the People's Republic of China

32170349G232402YDZX20223100001003

2024

10.1016/j.molp.2023.11.003

分子植物(英文版)
中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD
影响因子：0.659
ISSN：1674-2052
年,卷(期)：2024.17(1)
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