首页|A new oxidative pathway of nitric oxide production from oximes in plants

A new oxidative pathway of nitric oxide production from oximes in plants

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Nitric oxide(NO)is an essential reactive oxygen species and a signal molecule in plants.Although several studies have proposed the occurrence of oxidative NO production,only reductive routes for NO production,such as the nitrate(NO-3)-upper-reductase pathway,have been evidenced to date in land plants.However,plants grown axenically with ammonium as the sole source of nitrogen exhibit contents of nitrite and NO3-,evidencing the existence of a metabolic pathway for oxidative production of NO.We hypothesized that ox-imes,such as indole-3-acetaldoxime(IAOx),a precursor to indole-3-acetic acid,are intermediate oxidation products in NO synthesis.We detected the production of NO from IAOx and other oximes catalyzed by peroxidase(POD)enzyme using both 4-amino-5-methylamino-2',7'-difluorescein fluorescence and chem-iluminescence.Flavins stimulated the reaction,while superoxide dismutase inhibited it.Interestingly,mouse NO synthase can also use IAOx to produce NO at a lower rate than POD.We provided a full mech-anism for POD-dependent NO production from IAOx consistent with the experimental data and supported by density functional theory calculations.We showed that the addition of IAOx to extracts from Medicago truncatula increased the in vitro production of NO,while in vivo supplementation of IAOx and other oximes increased the number of lateral roots,as shown for NO donors,and a more than 10-fold increase in IAOx dehydratase expression.Furthermore,we found that in vivo supplementation of IAOx increased NO pro-duction in Arabidopsis thaliana wild-type plants,while prx33-34 mutant plants,defective in POD33-34,had reduced production.Our data show that the release of NO by IAOx,as well as its auxinic effect,explain the superroot phenotype.Collectively,our study reveals that plants produce NO utilizing diverse molecules such as oximes,POD,and flavins,which are widely distributed in the plant kingdom,thus intro-ducing a long-awaited oxidative pathway to NO production in plants.This knowledge has essential impli-cations for understanding signaling in biological systems.

ammonium oxidationauxinnitric oxideoximeperoxidaseroot phenotype

Pedro López-Gómez、Javier Buezo、Marina Urra、Alfonso Cornejo、Raquel Esteban、Jorge Fernández de los Reyes、Estibaliz Urarte、Estefanía Rodríguez-Dobreva、Alejandro Chamizo-Ampudia、Alejandro Eguaras、Sebastian Wolf、Daniel Marino、Victor Martínez-Merino、Jose F.Moran

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Institute for Multidisciplinary Research in Applied Biology(IMAB),Department of Sciences,Public University of Navarre(UPNA),Avda.de Pamplona 123,31192 Mutilva,Spain

Institute of Natural Resources and Agroblology of Salamanca(IRNASA),Department of Plant-Microorganism Interactions,Spanish National Research Council(CSIC),Cordel de Merinas,40-52,37008,Salamanca,Spain

Center for Plant Molecular Biology(ZMBP),University of Tübingen,Geschwister-Scholl-Platz,72074 Tübingen,Germany

Institute for Advanced Materials and Mathematics(INAMAT2),Department of Sciences,Public University of Navarre(UPNA),Campus de Arrosadía,31006 Pamplona,Spain

Department of Plant Biology and Ecology,University of the Basque Country(UPV/EHU),Sarriena s/n,Apdo.644,48080 Bilbao,Spain

Department of Biochemistry,Area of Molecular Biology,Veterinary Faculty,University of León,Campus de Vegazana,s/n.,24071 León,Spain

Centerfor Plant Molecular Biology(ZMBP),University of Tübingen,Geschwlster-Scholl-Platz,72074 Tübingen,Germany

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MCIN/AEI/10.13039/501100011033/FEDER,UEMCIN/AEI/10.13039/501100011033/FEDER,UEMCIN/AEI/10.13039/501100011033/FEDER,UEPublic University of NavarreUPV/EHU-GV IT-1018-16 program(Basque Government)Government of Navarre,SpainPublic University of Navarre,SpainSpanish National Research Councilthe"Requalification of the Spanish University System for 2021-2023,Public University of Navarra"fellowshipEuropean Union-Next Generation(EU)

AGL2014-52396AGL2017-86293-PPID2022-142968NB-100PID-2020-117703GB-I0020224AT017

2024

10.1016/j.molp.2023.12.009

分子植物(英文版)
中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD
影响因子：0.659
ISSN：1674-2052
年,卷(期)：2024.17(1)
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