Callus proliferation-induced hypoxic microenvironment decreases shoot regeneration competence in Arabidopsis

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外文摘要：Plants are aerobic organisms that rely on molecular oxygen for respiratory energy production.Hypoxic conditions,with oxygen levels ranging between 1％and 5％,usually limit aerobic respiration and affect plant growth and development.Here,we demonstrate that the hypoxic microenvironment induced by active cell proliferation during the two-step plant regeneration process intrinsically represses the regener-ation competence of the callus in Arabidopsis thaliana.We showed that hypoxia-repressed plant regener-ation is mediated by the RELATED TO APETALA 2.12(RAP2.12)protein,a member of the Ethylene Response Factor Ⅶ(ERF-Ⅶ)family.We found that the hypoxia-activated RAP2.12 protein promotes salicylic acid(SA)biosynthesis and defense responses,thereby inhibiting pluripotency acquisition and de novo shoot regeneration in calli.Molecular and genetic analyses revealed that RAP2.12 could bind directly to the SALICYLIC ACID INDUCTION DEFICIENT2(SID2)gene promoter and activate SA biosynthesis,repressing plant regeneration possibly via a PLETHORA(PLT)-dependent pathway.Consistently,the rap2.12 mutant calli exhibits enhanced shoot regeneration,which is impaired by SA treatment.Taken together,these find-ings uncover that the cell proliferation-dependent hypoxic microenvironment reduces cellular pluripotency and plant regeneration through the RAP2.12-SID2 module.

外文关键词：

calluscell proliferation-induced hypoxiaSID2plant regenerationsalicylic acidRAP2.12

作者：

Dohee Koo、Hong Gil Lee、Soon Hyung Bae、Kyounghee Lee、Pil Joon Seo

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作者单位：

Department of Chemistry,Seoul National University,Seoul 08826,Korea

Plant Genomics and Breeding Institute,Seoul National University,Seoul 08826,Korea

Research Institute of Basic Sciences,Seoul National University,Seoul 08826,Korea

基金：

Samsung Science and Technology Foundation(South Korea)New Breeding Technologies Development Program of the Rural Development Administration,South Korea

项目编号：

SSTF-BA2001-10RS-2024-00322275

出版年：

2024

DOI：

10.1016/j.molp.2024.01.009

分子植物(英文版)

中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD

影响因子：0.659

ISSN：1674-2052

年,卷(期)：2024.17(3)

参考文献量73