首页|Structures and ion transport mechanisms of plant high-affinity potassium transporters

Structures and ion transport mechanisms of plant high-affinity potassium transporters

扫码查看
原文链接
  • 万方数据
  • 维普
Plant high-affinity K+transporters(HKTs)mediate Na+and K+uptake,maintain Na+/K+homeostasis,and therefore play crucial roles in plant salt tolerance.In this study,we present cryoelectron microscopy structures of HKTs from two classes,class I HKT1;1 from Arabidopsis thaliana(AtHKT1;1)and class Ⅱ HKT2;1 from Triticum aestivum(TaHKT2;1),in both Na+-and K+-bound states at 2.6-to 3.0-Å resolutions.Both AtHKT1;1 and TaHKT2;1 function as homodimers.Each HKT subunit consists of four tan-dem domain units(D1-D4)with a repeated K+-channel-like M-P-M topology.In each subunit,D1-D4 assemble into an ion conduction pore with a pseudo-four-fold symmetry.Although both TaHKT2;1 and AtHKT1;1 have only one putative Na+ion bound in the selectivity filter with a similar coordination pattern,the two HKTs display different K+binding modes in the filter.TaHKT2;1 has three K+ions bound in the selec-tivity filter,but AtHKT1;1 has only two K+ions bound in the filter,which has a narrowed external entrance due to the presence of a Ser residue in the first filter motif.These structures,along with compu-tational,mutational,and electrophysiological analyses,enable us to pinpoint key residues that are critical for the ion selectivity of HKTs.The findings provide new insights into the ion selectivity and ion transport mechanisms of plant HKTs and improve our understanding about how HKTs mediate plant salt tolerance and enhance crop growth.

HKTsstructureion selectivitymechanismsalt tolerance

Jiangqin Wang、Yanping Luo、Fan Ye、Zhong Jie Ding、Shao Jian Zheng、Shuai Qiao、Yong Wang、Jiangtao Guo、Wei Yang、Nannan Su

展开 >

International Institutes of Medicine,the Fourth Affiliated Hospital,Zhejiang University School of Medicine,Yiwu,Zhejiang 322000,China

Department of Biophysics and Department of Neurology of the Fourth Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou,Zhejiang 310058,China

State Key Laboratory of Plant Environmental Resilience,College of Life Sciences,Zhejiang University,Hangzhou,Zhejiang 310058,China

College of Life Sciences,Zhejiang University,Hangzhou,Zhejiang 310058,China

NHC and CAMS Key Laboratory of Medical Neurobiology,MOE Frontier Science Center for Brain Science and Brain-machine Integration,School of Brain Science and Brain Medicine,Zhejiang University,Hangzhou,Zhejiang 310058,China

Na

展开 >

国家重点研发计划国家重点研发计划国家重点研发计划国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金浙江省自然科学基金中国博士后科学基金中国博士后科学基金National Postdoctoral Researcher Program of China中央高校基本科研业务费专项MOE Frontier Science Center for Brain Science & Brain-Machine Integration,Zhejiang UniversityInformation Technology Center and State Key Lab of CAD&CG at Zhejiang University

2022YFA13034002020YFA09085012021YFF120040432371204820301083187279632371300LR19C050002742023M743044GZB20230634

2024

10.1016/j.molp.2024.01.007

分子植物(英文版)
中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD
影响因子：0.659
ISSN：1674-2052
年,卷(期)：2024.17(3)
  • 71