High-quality assembled and annotated genomes of Nicotiana tabacum and Nicotiana benthamiana reveal chromosome evolution and changes in defense arsenals

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外文摘要：Nicotiana tabacum and Nicotiana benthamiana are widely used models in plant biology research.However,genomic studies of these species have lagged.Here we report the chromosome-level reference genome assemblies for N.benthamiana and N.tabacum with an estimated 99.5％and 99.8％completeness,respec-tively.Sensitive transcription start and termination site sequencing methods were developed and used for accurate gene annotation in N.tabacum.Comparative analyses revealed evidence for the parental origins and chromosome structural changes,leading to hybrid genome formation of each species.Interestingly,the antiviral silencing genes RDR1,RDR6,DCL2,DCL3,and AGO2 were lost from one or both subgenomes in N.benthamiana,while both homeologs were kept in N.tabacum.Furthermore,the N.benthamiana genome encodes fewer immune receptors and signaling components than that of N.tabacum.These find-ings uncover possible reasons underlying the hypersusceptible nature of N.benthamiana.We developed the user-friendly Nicomics(http://lifenglab.hzau.edu.cn/Nicomics/)web server to facilitate better use of Nicotiana genomic resources as well as gene structure and expression analyses.

外文关键词：

Nicotiana tabacumNicotiana benthamianagenomicsevolutiondisease resistanceNLR

作者：

Jubin Wang、Qingling Zhang、Jeffrey Tung、Xi Zhang、Dan Liu、Yingtian Deng、Zhendong Tian、Huilan Chen、Taotao Wang、Weixiao Yin、Bo Li、Zhibing Lai、Savithramma P.Dinesh-Kumar、Barbara Baker、Feng Li

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作者单位：

National Key Laboratory for Germplasm Innovation and Utilization for Fruit and Vegetable Horticultural Crops,College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan,Hubei 430070,China

The Key Laboratory of Horticultural Plant Genetic and Improvement of Jiangxi Province,Institute of Biological Resources,Jiangxi Academy of Sciences,Nanchang 330299,China

Institute of Vegetables and Flowers,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China

Plant Gene Expression Center,Department of Plant and Microbial Biology,University of California,Berkeley,Berkeley,CA 94706,USA

Hubei Hongshan Laboratory,Wuhan,Hubei 430070,China

College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China

College of Life Science and Technology,Huazhong Agricultural University,Wuhan,Hubei 430070,China

Department of Plant Biology and The Genome Center,College of Biological Sciences,University of California,Davis,Davis,CA 95616,USA

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基金：

国家自然科学基金国家自然科学基金国家自然科学基金USDA ARS CRISUSDA ARS CRISInnovative Genomics Institute(2017)

项目编号：

3227249132061143022322022502030-22000-009-00D2030-22000-034-00D

出版年：

2024

DOI：

10.1016/j.molp.2024.01.008

分子植物(英文版)

中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD

影响因子：0.659

ISSN：1674-2052

年,卷(期)：2024.17(3)

参考文献量59