首页|Systematic identification of wheat spike developmental regulators by integrated multi-omics,transcriptional network,GWAS,and genetic analyses

Systematic identification of wheat spike developmental regulators by integrated multi-omics,transcriptional network,GWAS,and genetic analyses

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The spike architecture of wheat plays a crucial role in determining grain number,making it a key trait for optimization in wheat breeding programs.In this study,we used a multi-omic approach to analyze the transcriptome and epigenome profiles of the young spike at eight developmental stages,revealing co-ordinated changes in chromatin accessibility and H3K27me3 abundance during the flowering transition.We constructed a core transcriptional regulatory network(TRN)that drives wheat spike formation and experimentally validated a multi-layer regulatory module involving TaSPL15,TaAGLG1,and TaFUL2.By integrating the TRN with genome-wide association studies,we identified 227 transcription factors,including 42 with known functions and 185 with unknown functions.Further investigation of 61 novel transcription factors using multiple homozygous mutant lines revealed 36 transcription factors that regulate spike architecture or flowering time,such as TaMYC2-A1,TaMYB30-A1,and TaWRKY37-A1.Of particular interest,TaMYB30-A1,downstream of and repressed by WFZP,was found to regulate fertile spikelet number.Notably,the excellent haplotype of TaMYB30-A1,which contains a C allele at the WFZP binding site,was enriched during wheat breeding improvement in China,leading to improved agronomic traits.Finally,we constructed a free and open access Wheat Spike Multi-Omic Database(http://39.98.48.156:8800/#/).Our study identifies novel and high-confidence regulators and offers an effective strategy for dissecting the genetic basis of wheat spike development,with practical value for wheat breeding.

wheat spike developmentepigenomeTRNTaMYB30-A1breeding selection

Xuelei Lin、Yongxin Xu、Dongzhi Wang、Yiman Yang、Xiaoyu Zhang、Xiaomin Bie、Lixuan Gui、Zhongxu Chen、Yiliang Ding、Long Mao、Xueyong Zhang、Fei Lu、Xiansheng Zhang、Cristobal Uauy、Xiangdong Fu、Jun Xiao

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Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China

University of Chinese Academy of Sciences,Beijing 100049,China

Nanjing Agricultural University,Nanjing,Jiangsu 210095,China

Key Laboratory of Crop Biology,College of Life Sciences,Shandong Agricultural University,Tai'an,Shandong 271018,China

Department of Life Science,Tcuni Inc.,Chengdu,Sichuan 610000,China

John Innes Centre,Norwich Research Park,Norwich NR4 7UH,UK

Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

CAS-JIC Centre of Excellence for Plant and Microbial Science(CEPAMS),Institute of Genetics and Developmental Biology,CAS,Beijing 100101,China

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国家自然科学基金中国科学院战略规划重点项目国家重点研发计划Major Basic Research Program of Shandong Natural Science Foundation of China

31921005XDA240102042021YFD1201500ZR2019ZD15

2024

10.1016/j.molp.2024.01.010

分子植物(英文版)
中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD
影响因子：0.659
ISSN：1674-2052
年,卷(期)：2024.17(3)
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