Phase separation of GRP7 facilitated by FERONIA-mediated phosphorylation inhibits mRNA translation to modulate plant temperature resilience

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外文摘要：Changes in ambient temperature profoundly affect plant growth and performance.Therefore,the molecu-lar basis of plant acclimation to temperature fluctuation is of great interest.In this study,we discovered that GLYCINE-RICH RNA-BINDING PROTEIN 7(GRP7)contributes to cold and heat tolerance in Arabidopsis thaliana.We found that exposure to a warm temperature rapidly induces GRP7 condensates in planta,which can be reversed by transfer to a lower temperature.Cell biology and biochemical assays revealed that GRP7 undergoes liquid-liquid phase separation(LLPS)in vivo and in vitro.LLPS of GRP7 in the cyto-plasm contributes to the formation of stress granules that recruit RNA,along with the translation machinery component eukaryotic initiation factor 4E1(eIF4E1)and the mRNA chaperones COLD SHOCK PROTEIN 1(CSP1)and CSP3,to inhibit translation.Moreover,natural variations in GRP7 affecting the residue phos-phorylated by the receptor kinase FERONIA alter its capacity to undergo LLPS and correlate with the adap-tation of some Arabidopsis accessions to a wider temperature range.Taken together,our findings illustrate the role of translational control mediated by GRP7 LLPS to confer plants with temperature resilience.

外文关键词：

fluctuating temperatureglycine-rich RNA-binding proteinphase separationcold shock proteinstranslation

作者：

Fan Xu、Long Wang、Yingbin Li、Junfeng Shi、Dorothee Staiger、Feng Yu

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作者单位：

State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Biology,and Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation,Hunan University,Changsha 410082,P.R.China

State Key Laboratory of Hybrid Rice,Hunan Agricultural Biotechnology Research Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,P.R.China

RNA Biology and Molecular Physiology,Faculty of Biology,Bielefeld University,33615 Bielefeld,Germany

基金：

国家自然科学基金国家自然科学基金国家重点研发计划中国博士后科学基金Science and Technology Innovation Program of Hunan ProvinceScience and Technology Innovation Program of Hunan ProvinceScience and Technology Innovation Program of Hunan ProvinceScience and Technology Innovation Program of Hunan Province

项目编号：

NSFC-32000208NSFC-320707692023YFD14011002020M6724752021JJ100152021JJ400602023ZJ10802021JJ40056

出版年：

2024

DOI：

10.1016/j.molp.2024.02.001

分子植物(英文版)

中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD

影响因子：0.659

ISSN：1674-2052

年,卷(期)：2024.17(3)

参考文献量77