Dt1 inhibits SWEET-mediated sucrose transport to regulate photoperiod-dependent seed weight in soybean

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外文摘要：Soybean is a photoperiod-sensitive short-day crop whose reproductive period and yield are markedly affected by day-length changes.Seed weight is one of the key traits determining the soybean yield;how-ever,the prominent genes that control the final seed weight of soybean and the mechanisms underlying the photoperiod's effect on this trait remain poorly understood.In this study,we identify SW19 as a major locus controlling soybean seed weight by QTL mapping and determine Dt1,an orthologous gene of Arabidopsis TFL1 that is known to govern the soybean growth habit,as the causal gene of the SW19 locus.We showed that Dt1 is highly expressed in developing seeds and regulates photoperiod-dependent seed weight in soybean.Further analyses revealed that the Dt1 protein physically interacts with the sucrose transporter GmSWEET10a to negatively regulate the import of sucrose from seed coat to the embryo,thus modulating seed weight under long days.However,Dt1 does not function in seed development under short days due to its very low expression.Importantly,we discovered a novel natural allelic variant of Dt1(H4 haplotype)that decouples its pleiotropic effects on seed size and growth habit;i.e.,this variant remains functional in seed development but fails to regulate the stem growth habit of soybean.Collectively,our findings provide new insights into how soybean seed development responds to photoperiod at different latitudes,offering an ideal genetic component for improving soybean's yield by manipulating its seed weight and growth habit.

外文关键词：

soybeanseed weightphotoperioddt1sucrose transport

作者：

Xiaoming Li、Zhonghui Chen、Haiyang Li、Lin Yue、Cuirong Tan、Hongjie Liu、Yilong Hu、Yuhua Yang、Xiani Yao、Lingping Kong、Xiang Huang、Bin Yu、Chunyu Zhang、Yuefeng Guan、Baohui Liu、Fanjiang Kong、Xingliang Hou

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作者单位：

Guangdong Provincial Key Laboratory of Applied Botany & State Key Laboratory of Plant Diversity and Specialty Crops,South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China

University of Chinese Academy of Sciences,Beijing 100049,China

Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design,Guangzhou Key Laboratory of Crop Gene Editing,Innovative Center of Molecular Genetics and Evolution,School of Life Sciences,Guangzhou University,Guangzhou 510006,China

College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China

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基金：

国家自然科学基金国家重点研发计划中国科学院战略规划重点项目国家自然科学基金国家自然科学基金Major Program of Guangdong Basic and Applied ResearchGuangzhou Municipal Science and Technology Project

项目编号：

320900642021YFF1001203XDA2401010532230078318716432019B030302006202201010641

出版年：

2024

DOI：

10.1016/j.molp.2024.02.007

分子植物(英文版)

中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD

影响因子：0.659

ISSN：1674-2052

年,卷(期)：2024.17(3)

参考文献量51