Two gene clusters and their positive regulator SIMYB13 that have undergone domestication-associated negative selection control phenolamide accumulation and drought tolerance in tomato

扫码查看

原文链接

万方数据

外文摘要：Among plant metabolites,phenolamides,which are conjugates of hydroxycinnamic acid derivatives and polyamines,play important roles in plant adaptation to abiotic and biotic stresses.However,the molecular mechanisms underlying phenolamide metabolism and regulation as well as the effects of domestication and breeding on phenolamide diversity in tomato remain largely unclear.In this study,we performed a metabolite-based genome-wide association study and identified two biosynthetic gene clusters(BGC7 and BGC11)containing 12 genes involved in phenolamide metabolism,including four biosynthesis genes(two 4CL genes,one C3H gene,and one CPA gene),seven decoration genes(five AT genes and two UGT genes),and one transport protein gene(DTX29).Using gene co-expression network analysis we further discovered that SIMYB13 positively regulates the expression of two gene clusters,thereby promoting phe-nolamide accumulation.Genetic and physiological analyses showed that BGC7,BGC11 and SIMYB13 enhance drought tolerance by enhancing scavenging of reactive oxygen species and increasing abscisic acid content in tomato.Natural variation analysis suggested that BGC7,BGC11 and SIMYB13 were nega-tively selected during tomato domestication and improvement,leading to reduced phenolamide content and drought tolerance of cultivated tomato.Collectively,our study discovers a key mechanism of phenolamide biosynthesis and regulation in tomato and reveals that crop domestication and improve-ment shapes metabolic diversity to affect plant environmental adaptation.

外文关键词：

tomatophenolamidesgene clusterdrought tolerancenatural variationevolution

作者：

Peng Cao、Jun Yang、Linghao Xia、Zhonghui Zhang、Zeyong Wu、Yingchen Hao、Penghui Liu、Chao Wang、Chun Li、Jie Yang、Jun Lai、Xianggui Li、Meng Deng、Shouchuang Wang

展开 >

作者单位：

School of Breeding and Multiplication(Sanya Institute of Breeding and Multiplication),Hainan University,Sanya 572025,China

College of Tropical Agriculture and Forestry,Hainan University,Haikou 572208,China

Collaborative Innovation Center of Nanfan and High-Efficiency Tropical Agriculture,Hainan University,Haikou 572208,China

基金：

国家重点研发计划Hainan Province Science and Technology Special FundHainan Provincial Academician Innovation Platform ProjectYoung Elite Scientists Sponsorship Program by China Association for Science and TechnologyHainan University Startup FundCollaborative Innovation Center of Nanfan and High-Efficiency Tropical Agriculture,Hainan UniversityInnovation Project of Postgraduates of Hainan Province

项目编号：

2022YFF1001900ZDYF2022XDNY144HD-YSZX-2020042019QNRC001KYQDZR21025XTCX2022NYB06Qhyb2022-56

出版年：

2024

DOI：

10.1016/j.molp.2024.02.003

分子植物(英文版)

中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD

影响因子：0.659

ISSN：1674-2052

年,卷(期)：2024.17(4)

参考文献量3