The RNA binding protein EHD6 recruits the m6A reader YTH07 and sequesters OsCOL4 mRNA into phase-separated ribonucleoprotein condensates to promote rice flowering

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外文摘要：N6-Methyladenosine(m6A)is one of the most abundant modifications of eukaryotic mRNA,but its compre-hensive biological functionality remains further exploration.In this study,we identified and characterized a new flowering-promoting gene,EARLY HEADING DATE6(EHD6),in rice.EHD6 encodes an RNA recognition motif(RRM)-containing RNA binding protein that is localized in the non-membranous cytoplasm ribonu-cleoprotein(RNP)granules and can bind both m6A-modified RNA and unmodified RNA indiscriminately.We found that EHD6 can physically interact with YTH07,a YTH(YT521-B homology)domain-containing m6A reader.We showed that their interaction enhances the binding of an m6A-modified RNA and triggers relocation of a portion of YTH07 from the cytoplasm into RNP granules through phase-separated conden-sation.Within these condensates,the mRNA of a rice flowering repressor,CONSTANS-like 4(OsCOL4),be-comes sequestered,leading to a reduction in its protein abundance and thus accelerated flowering through the Early heading date 1 pathway.Taken together,these results not only shed new light on the molecular mechanism of efficient m6A recognition by the collaboration between an RNA binding protein and YTH fam-ily m6A reader,but also uncover the potential for m6A-mediated translation regulation through phase-separated ribonucleoprotein condensation in rice.

外文关键词：

N6-methyladenosinem6AEHD6YTH domain-containing proteinphase separationheading date

作者：

Song Cui、Peizhe Song、Chaolong Wang、Saihua Chen、Benyuan Hao、Zhuang Xu、Liang Cai、Xu Chen、Shanshan Zhu、Xiangchao Gan、Hui Dong、Yuan Hu、Liang Zhou、Haigang Hou、Yunlu Tian、Xi Liu、Liangming Chen、Shijia Liu、Ling Jiang、Haiyang Wang、Guifang Jia、Shirong Zhou、Jianmin Wan

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作者单位：

State Key Laboratory for Crop Genetics & Germplasm Enhancement and Utilization,Zhongshan Biological Breeding Laboratory,National Observation and Research Station of Rice Germplasm Resources,Nanjing Agricultural University,Nanjing 210095,China

State Key Laboratory for Crop Gene Resources and Breeding,Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China

Synthetic and Functional Biomolecules Center,Beijing National Laboratory for Molecular Sciences,Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education,College of Chemistry and Molecular Engineering,Peking-Tsinghua Center for Life Sciences,Beijing Advanced Center of RNA Biology,Peking University,Beijing,China

Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding,Agricultural College of Yangzhou University,Yangzhou 225009,China

Max Planck Institute for Plant Breeding Research,Carl-von-Linné-Weg 10,50829 Köln,Germany

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基金：

Key Laboratory of Biology,Genetics and Breeding of Japonica Rice in Midlower Yangtze River,Ministry of Agriculture and RuralJiangsu Collaborative Innovation Center for Modern Crop Production,ChinaNational Key Research and Development Program of ChinaNational Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Science Foundation of Jiangsu ProvinceNational Science Foundation of Jiangsu ProvinceFoundation of Biological Breeding Zhongshan LabFoundation of Biological Breeding Zhongshan LabFoundation of Biological Breeding Zhongshan Lab

项目编号：

2020YFE02023002021YFD12005043197191032272115BK20212010BK20230038BM2022008-03ZSBBL-KY2023-04ZSBBL-KY2023-06

出版年：

2024

DOI：

10.1016/j.molp.2024.05.002

分子植物(英文版)

中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD

影响因子：0.659

ISSN：1674-2052

年,卷(期)：2024.17(6)