首页|A circular single-stranded DNA mycovirus infects plants and confers broad-spectrum fungal resistance

A circular single-stranded DNA mycovirus infects plants and confers broad-spectrum fungal resistance

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Circular single-stranded DNA(ssDNA)viruses have been rarely found in fungi,and the evolutionary and ecological relationships among ssDNA viruses infecting fungi and other organisms remain unclear.In this study,a novel circular ssDNA virus,tentatively named Diaporthe sojae circular DNA virus 1(DsCDV1),was identified in the phytopathogenic fungus Diaporthe sojae isolated from pear trees.DsCDV1 has a monopartite genome(3185 nt in size)encapsidated in isometric virions(21-26 nm in diam-eter).The genome comprises seven putative open reading frames encoding a discrete replicase(Rep)split by an intergenic region,a putative capsid protein(CP),several proteins of unknown function(P1-P4),and a long intergenic region.Notably,the two split parts of DsCDV1 Rep share high identities with the Reps of Geminiviridae and Genomoviridae,respectively,indicating an evolutionary linkage with both families.Phylogenetic analysis based on Rep or CP sequences placed DsCDV1 in a unique cluster,supporting the establishment of a new family,tentatively named Gegemycoviridae,intermediate to both families.DsCDV1 significantly attenuates fungal growth and nearly erases fungal virulence when transfected into the host fungus.Remarkably,DsCDV1 can systematically infect tobacco and pear seedlings,providing broad-spectrum resistance to fungal diseases.Subcellular localization analysis revealed that DsCDV1 P3 is systematically localized in the plasmodesmata,while its expression in trans-complementation experi-ments could restore systematic infection of a movement-deficient plant virus,suggesting that P3 is a move-ment protein.DsCDV1 exhibits unique molecular and biological traits not observed in other ssDNA viruses,serving as a link between fungal and plant ssDNA viruses and presenting an evolutionary connection be-tween ssDNA viruses and fungi.These findings contribute to expanding our understanding of ssDNA virus diversity and evolution,offering potential biocontrol applications for managing crucial plant diseases.

circular single-stranded DNA virusmycovirusvirus evolutionGenomoviridaeGegemycoviridaeDi-aporthe sojae circular DNA virus 1

Xianhong Wang、Ioly Kotta-Loizou、Robert H.A.Coutts、Huifang Deng、Zhenhao Han、Ni Hong、Karim Shafik、Liping Wang、Yashuang Guo、Mengmeng Yang、Wenxing Xu、Guoping Wang

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National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops,Wuhan 430070,China

Hubei Hongshan Laboratory,Wuhan 430070,China

College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China

Key Lab of Plant Pathology of Hubei Province,Wuhan 430070,China

Department of Life Sciences,Faculty of Natural Sciences,Imperial College London,London SW7 2AZ,UK

Department of Clinical,Pharmaceutical and Biological Science,School of Life and Medical Sciences,University of Hertfordshire,Hatfield AL10 9AB,UK

Department of Plant Pathology,Faculty of Agriculture,Alexandria University,Alexandria 21526,Egypt

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Earmarked Fund for China Agricultural Research SystemNational Natural Science Foundation of China

CARS-2832172475

2024

10.1016/j.molp.2024.05.003

分子植物(英文版)
中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD
影响因子：0.659
ISSN：1674-2052
年,卷(期)：2024.17(6)