Non-transcriptional regulatory activity of SMAX1 and SMXL2 mediates karrikin-regulated seedling response to red light in Arabidopsis

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外文摘要：Karrikins and strigolactones govern plant development and environmental responses through closely related signaling pathways.The transcriptional repressor proteins SUPPRESSOR OF MAX2 1(SMAX1),SMAX1-like2(SMXL2),and D53-like SMXLs mediate karrikin and strigolactone signaling by directly binding downstream genes or by inhibiting the activities of transcription factors.In this study,we characterized the non-transcriptional regulatory activities of SMXL proteins in Arabidopsis.We discovered that SMAX1 and SMXL2 with mutations in their ethylene-response factor-associated amphiphilic repression(EAR)motif had undetectable or weak transcriptional repression activities but still partially rescued the hypocotyl elonga-tion defects and fully reversed the cotyledon epinasty defects of the smax1 smxl2 mutant.SMAX1 and SMXL2 directly interact with PHYTOCHROME INTERACTION FACTOR 4(PIF4)and PIF5 to enhance their protein stability by interacting with phytochrome B(phyB)and suppressing the association of phyB with PIF4 and PIF5.The karrikin-responsive genes were then identified by treatment with GR24ent-5DS,a GR24 analog showing karrikin activity.Interestingly,INDOLE-3-ACETIC ACID INDUCIBLE 29(IAA29)expression was repressed by GR24ent-5DS treatment in a PIF4-and PIF5-dependent and EAR-independent manner,whereas KARRIKIN UPREGULATED F-BOX 1(KUF1)expression was induced in a PIF4-and PIF5-independent and EAR-dependent manner.Furthermore,the non-transcriptional regulatory activity of SMAX1,which is independent of the EAR motif,had a global effect on gene expression.Taken together,these results indicate that non-transcriptional regulatory activities of SMAX1 and SMXL2 mediate karrikin-regulated seedling response to red light.

外文关键词：

karrikin signalingSMAX1 and SMXL2non-transcriptional repression activityPIF4 and PIF5gene expressionred light

作者：

Wenwen Chang、Qiao Qiao、Qingtian Li、Xin Li、Yanyan Li、Xiahe Huang、Yingchun Wang、Jiayang Li、Bing Wang、Lei Wang

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作者单位：

Key Laboratory of Seed Innovation,Center for Agricultural Resources Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Shijiazhuang,Hebei 050021,China

University of Chinese Academy of Sciences,Beijing 100049,China

Yazhouwan National Laboratory,Sanya,Hainan 572024,China

Key Laboratory of Seed Innovation,Institute of Genetics and Developmental Biology,The Innovative Academy for Seed Design,Chinese Academy of Sciences,Beijing 100101,China

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基金：

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaHebei Natural Science FoundationYouth Innovation Promotion Association of the Chinese Academy of Sciences

项目编号：

321703203212201232270327C2022503003Y2023025

出版年：

2024

DOI：

10.1016/j.molp.2024.05.007

分子植物(英文版)

中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD

影响因子：0.659

ISSN：1674-2052

年,卷(期)：2024.17(7)