Dynamic modulation of nodulation factor receptor levels by phosphorylation-mediated functional switch of a RING-type E3 ligase during legume nodulation

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外文摘要：The precise control of receptor levels is crucial for initiating cellular signaling transduction in response to specific ligands;however,such mechanisms regulating nodulation factor(NF)receptor(NFR)-mediated perception of NFs to establish symbiosis remain unclear.In this study,we unveil the pivotal role of the NFR-interacting RING-type E3 ligase 1(NIRE1)in regulating NFR1/NFR5 homeostasis to optimize rhizobial infection and nodule development in Lotus japonicus.We demonstrated that NIRE1 has a dual function in this regulatory process.It associates with both NFR1 and NFR5,facilitating their degradation through K48-linked polyubiquitination before rhizobial inoculation.However,following rhizobial inoculation,NFR1 phosphorylates NIRE1 ata conserved residue,Tyr-109,inducing afunctional switch in NIRE1,which enables NIRE1 to mediate K63-linked polyubiquitination,thereby stabilizing NFR1/NFR5 in infected root cells.The introduction of phospho-dead NIRE1Y109F leads to delayed nodule development,underscoring the signifi-cance of phosphorylation at Tyr-109 in orchestrating symbiotic processes.Conversely,expression of the phospho-mimic NIRE1Y109E results in the formation of spontaneous nodules in L.japonicus,further empha-sizing the critical role of the phosphorylation-dependent functional switch in NIRE1.In summary,these find-ings uncover a fine-tuned symbiotic mechanism that a single E3 ligase could undergo a phosphorylation-dependent functional switch to dynamically and precisely regulate NF receptor protein levels.

外文关键词：

nodule symbiosisnodulation factor receptorsE3 ligaseubiquitinationphosphorylationprotein degradationprotein stabilizationLotus japonicus

作者：

Hao Li、Yajuan Ou、Jidan Zhang、Kui Huang、Ping Wu、Xiaoli Guo、Hui Zhu、Yangrong Cao

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作者单位：

National Key Lab of Agricultural Microbiology,Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070,China

National Key Lab of Agricultural Microbiology,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,China

基金：

National Key R&D Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaChina Postdoctoral Science FoundationSelf-Innovation grant from National Laboratory

项目编号：

2019YFA090470032200207320900632019M662652AML2023B01

出版年：

2024

DOI：

10.1016/j.molp.2024.05.010

分子植物(英文版)

中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD

影响因子：0.659

ISSN：1674-2052

年,卷(期)：2024.17(7)

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