首页|CONSTANS alters the circadian clock in Arabidopsis thaliana
CONSTANS alters the circadian clock in Arabidopsis thaliana
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Plants are sessile organisms that have acquired highly plastic developmental strategies to adapt to the environment.Among these processes,the floral transition is essential to ensure reproductive success and is finely regulated by several internal and external genetic networks.The photoperiodic pathway,which controls plant response to day length,is one of the most important pathways controlling flowering.In Ara-bidopsis photoperiodic flowering,CONSTANS(CO)is the central gene activating the expression of the florigen FLOWERING LOCUS T(FT)in the leaves at the end of a long day.The circadian clock strongly reg-ulates CO expression.However,to date,no evidence has been reported regarding a feedback loop from the photoperiod pathway back to the circadian clock.Using transcriptional networks,we have identified rele-vant network motifs regulating the interplay between the circadian clock and the photoperiod pathway.Gene expression,chromatin immunoprecipitation experiments,and phenotypic analysis allowed us to elucidate the role of CO over the circadian clock.Plants with altered CO expression showed a different in-ternal clock period,measured by daily leaf rhythmic movements.We showed that CO upregulates the expression of key genes related to the circadian clock,such as CCA 1,LHY,PRR5,and GI,at the end of a long day by binding to specific sites on their promoters.Moreover,a high number of PRR5-repressed target genes are upregulated by CO,and this could explain the phase transition promoted by CO.The CO-PRR5 complex interacts with the bZIP transcription factor HY5 and helps to localize the complex in the promoters of clock genes.Taken together,our results indicate that there may be a feedback loop in which CO com-municates back to the circadian clock,providing seasonal information to the circadian system.
CONSTANScircadian clockphotoperiod
Pedro de los Reyes、Gloria Serrano-Bueno、Francisco J.Romero-Campero、He Gao、Jose M.Romero、Federico Valverde
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Plant Development Group-Institute for Plant Biochemistry and Photosynthesis,Consejo Superior de Investigaciones Científicas,Universidad de Sevilla,Seville,Spain
Department of Plant Biochemistry and Molecular Biology,Universidad de Sevilla,Seville,Spain
Department of Computer Science and Artificial Intelligence,Universidad de Sevilla,Seville,Spain
Department of Plant Developmental Biology,Max Planck Institute for Plant Breeding Research,Cologne,Germany
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European Union contract LONGFLOWCSIC LONGFLOWSpanish Ministry for Science and Innovations(MICINN/FEDER)
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