Structural variations of a new fertility restorer gene,Rf20,underlie the restoration of wild abortive-type cytoplasmic male sterility in rice

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外文摘要：The discovery of a wild abortive-type(WA)cytoplasmic male sterile(CMS)line and breeding its restorer line have led to the commercialization of three-line hybrid rice,contributing considerably to global food security.However,the molecular mechanisms underlying fertility abortion and the restoration of CMS-WA lines remain largely elusive.In this study,we cloned a restorer gene,Rf20,following a genome-wide association study analysis of the core parent lines of three-line hybrid rice.We found that Rf20 was present in all core parental lines,but different haplotypes and structural variants of its gene resulted in differences in Rf20 expression levels between sterile and restored lines.Rf20 could restore pollen fertility in the CMS-WA line and was found to be responsible for fertility restoration in some CMS lines under high temperatures.In addition,we found that Rf20 encodes a pentatricopeptide repeat protein that competes with WA352 for binding with COX11.This interaction enhances COX11's function as a scavenger of reactive oxygen species,which in turn restores pollen fertility.Collectively,our study suggests a new action mode for pentatricopeptide repeat proteins in the fertility restoration of CMS lines,providing an essential theoretical basis for breeding robust restorer lines and for overcoming high temperature-induced fertility recovery of some CMS lines.

外文关键词：

COX11cytoplasmic male sterilityrf20WA352wild abortion

作者：

Shufeng Song、Yixing Li、Mudan Qiu、Na Xu、Bin Li、Longhui Zhang、Lei Li、Weijun Chen、Jinglei Li、Tiankang Wang、Yingxin Qiu、Mengmeng Gong、Dong Yu、Hao Dong、Siqi Xia、Yi Pan、Dingyang Yuan、Li Li

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作者单位：

State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Hunan Academy of Agricultural Sciences,Changsha 410125,China

College of Agronomy,Hunan Agricultural University,Changsha 410128,China

College of Plant Protection,Hunan Agricultural University,Changsha 410128,China

College of Tropical Crops,Hainan University,Haikou 570228,China

Longping Branch,College of Biology,Hunan University,Changsha 410125,China

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基金：

Biological Breeding-Major ProjectsNational Key Research and Development ProjectHunan Natural Science FoundationSpecial Funds for the Construction of Innovative Provinces in Hunan Province

项目编号：

2023ZD040762022YFD12008002023JJ304422021NK1002

出版年：

2024

DOI：

10.1016/j.molp.2024.07.001

分子植物(英文版)

中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD

影响因子：0.659

ISSN：1674-2052

年,卷(期)：2024.17(8)