首页|Hordedane diterpenoid phytoalexins restrict Fusarium graminearum infection but enhance Bipolaris sorokiniana colonization of barley roots

Hordedane diterpenoid phytoalexins restrict Fusarium graminearum infection but enhance Bipolaris sorokiniana colonization of barley roots

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Plant immunity is a multilayered process that includes recognition of patterns or effectors from pathogens to elicit defense responses.These include the induction of a cocktail of defense metabolites that typically restrict pathogen virulence.Here,we investigate the interaction between barley roots and the fungal path-ogens Bipolaris sorokiniana(Bs)and Fusarium graminearum(Fg)at the metabolite level.We identify horde-danes,a previously undescribed set of labdane-related diterpenoids with antimicrobial properties,as crit-ical players in these interactions.Infection of barley roots by Bs and Fg elicits hordedane synthesis from a 600-kb gene cluster.Heterologous reconstruction of the biosynthesis pathway in yeast and Nicotiana ben-thamiana produced several hordedanes,including one of the most functionally decorated products 19-β-hydroxy-hordetrienoic acid(19-OH-HTA).Barley mutants in the diterpene synthase genes of this cluster are unable to produce hordedanes but,unexpectedly,show reduced Bs colonization.By contrast,coloni-zation by Fusarium graminearum,another fungal pathogen of barley and wheat,is 4-fold higher in the mu-tants completely lacking hordedanes.Accordingly,19-OH-HTA enhances both germination and growth of Bs,whereas it inhibits other pathogenic fungi,including Fg.Analysis of microscopy and transcriptomics data suggest that hordedanes delay the necrotrophic phase of Bs.Taken together,these results show that adapted pathogens such as Bs can subvert plant metabolic defenses to facilitate root colonization.

barleyHordeum vulgarediterpenoid phytoalexinsgene clusterpathogenic fungiBipolaris soroki-ninianaFusarium graminearum

Yaming Liu、Dario Esposto、Lisa K.Mahdi、Andrea Porzel、Pauline Stark、Hidayat Hussain、Anja Scherr-Henning、Simon Isfort、Ulschan.Bathe、Iván F.Acosta、Alga Zuccaro、Gerd U.Balcke、Alain Tissier

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Department of Cell and Metabolic Biology,Leibniz Institute of Plant Biochemistry,Halle,Germany

Institute for Plant Sciences,Cluster of Excellence on Plant Sciences(CEPLAS),Cologne Biocenter,University of Cologne,Cologne,Germany

Department of Bioorganic Chemistry,Leibniz Institute of Plant Biochemistry,Halle,Germany

Horticultural Sciences,University of Florida,Gainesville,FL,USA

Max Planck Institute for Plant Breeding Research,Cologne,Germany

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Deutsche Forschungsgemeinschaft,GermanyDeutsche Forschungsgemeinschaft,GermanyCluster of Excellence on Plant Sciences(CEPLAS)funded by the Deutsche Forschungsgemeinschaft under Germany's Excellence Stra&&

TI 800/7-1TI 800/7-2SPP 2125 DECRyPTEXC 2048/1-390686111ZU 263/11-2SPP 2125 DECRyPT

2024

10.1016/j.molp.2024.07.006

分子植物(英文版)
中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD
影响因子：0.659
ISSN：1674-2052
年,卷(期)：2024.17(8)
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