首页|The OsSRO1c-OsDREB2B complex undergoes protein phase transition to enhance cold tolerance in rice

The OsSRO1c-OsDREB2B complex undergoes protein phase transition to enhance cold tolerance in rice

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Cold stress is one of the major abiotic stress factors affecting rice growth and development,leading to significant yield loss in the context of global climate change.Exploring natural variants that confer cold resistance and the underlying molecular mechanism responsible for this is the major strategy to breed cold-tolerant rice varieties.Here,we show that natural variations of a SIMILAR to RCD ONE(SRO)gene,OsSRO1c,confer cold tolerance in rice at both seedling and booting stages.Our in vivo and in vitro exper-iments demonstrated that OsSRO1c possesses intrinsic liquid-liquid phase-separation ability and recruits OsDREB2B,an AP2/ERF transcription factor that functions as a positive regulator of cold stress,into its biomolecular condensates in the nucleus,resulting in elevated transcriptional activity of OsDREB2B.We found that the OsSRO1 c-OsDREB2B complex directly responds to low temperature through dynamic phase transitions and regulates key cold-response genes,including COLD1.Furthermore,we showed that intro-gression of an elite haplotype of OsSRO1c into a cold-susceptible indica rice could significantly increase its cold resistance.Collectively,our work reveals a novel cold-tolerance regulatory module in rice and pro-vides promising genetic targets for molecular breeding of cold-tolerant rice varieties.

cold-stress sensingnatural variationcrop genetic improvementliquid-liquid phase separationclimate change

Dan Hu、Yilong Yao、Yan Lv、Jun You、Yu Zhang、Qingya Lv、Jiawei Li、Stephanie Hutin、Haiyan Xiong、Chloe Zubieta、Xuelei Lai、Lizhong Xiong

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National Key Laboratory of Crop Genetic Improvement,Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan,China

Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture,Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences,Wuhan,China

Laboratoire de Physiologie Cellulaire et Végétale,Université Grenoble-Alpes,CNRS,CEA,INRAE,IRIG-DBSCI,38000 Grenoble,France

2024

10.1016/j.molp.2024.08.006

分子植物(英文版)
中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD
影响因子：0.659
ISSN：1674-2052
年,卷(期)：2024.17(10)