首页|Architecture of the ATP-driven motor for protein import into chloroplasts

Architecture of the ATP-driven motor for protein import into chloroplasts

扫码查看
原文链接
  • 万方数据
  • 维普
Thousands of nuclear-encoded proteins are transported into chloroplasts through the TOC-TIC translocon that spans the chloroplast envelope membranes.A motor complex pulls the translocated proteins out of the TOC-TIC complex into the chloroplast stroma by hydrolyzing ATP.The Orf2971-FtsHi complex has been suggested to serve as the ATP-hydrolyzing motor in Chlamydomonas reinhardtii,but little is known about its architecture and assembly.Here,we report the 3.2-Å resolution structure of the Chlamydomonas Orf2971-FtsHi complex.The 20-subunit complex spans the chloroplast inner envelope,with two bulky modules protruding into the intermembrane space and stromal matrix.Six subunits form a hetero-hexamer that potentially provides the pulling force through ATP hydrolysis.The remaining subunits,including potential enzymes/chaperones,likely facilitate the complex assembly and regulate its proper function.Taken together,our results provide the structural foundation for a mechanistic understanding of chloroplast protein translocation.

chloroplast protein translocationcryo-EM structureOrf2971-FtsHiATP-driven motorChlamydomonas

Ning Wang、Jiale Xing、Xiaodong Su、Junting Pan、Hui Chen、Lifang Shi、Long Si、Wenqiang Yang、Mei Li

展开 >

Key Laboratory of Biomacromolecules(CAS),National Laboratory of Biomacromolecules,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China

Photosynthesis Research Center,Key Laboratory of Photobiology,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China

University of Chinese Academy of Sciences,Beijing,China

China National Botanical Garden,Beijing 100093,China

Institute of Botany,Chinese Academy of Sciences,Beijing,China

Key Laboratory of Epigenetic Regulation and Intervention,Chinese Academy of Sciences Center for Excellence in Molecular Cell Science,Shanghai Institute of Biochemistry and Cell Biology,Chinese Academy of Sciences,Shanghai 200031,China

展开 >

2024

10.1016/j.molp.2024.09.010

分子植物(英文版)
中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD
影响因子：0.659
ISSN：1674-2052
年,卷(期)：2024.17(11)