首页|The metal tolerance protein OsMTP11 facilitates cadmium sequestration in the vacuoles of leaf vascular cells for restricting its translocation into rice grains

The metal tolerance protein OsMTP11 facilitates cadmium sequestration in the vacuoles of leaf vascular cells for restricting its translocation into rice grains

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Rice(Oryza sativa)provides＞20％of the consumed calories in the human diet.However,rice is also a lead-ing source of dietary cadmium(Cd)that seriously threatens human health.Deciphering the genetic network that underlies the grain-Cd accumulation will benefit the development of low-Cd rice and mitigate the ef-fects of Cd accumulation in the rice grain.In this study,we identified a QTL gene,OsCS1,which is allelic to OsMTP11 and encodes a protein sequestering Cd in the leaf during vegetative growth and preventing Cd from being translocated to the grain after heading in rice.OsCS1 is predominantly expressed in leaf vascular parenchyma cells,where it binds to a vacuole-sorting receptor protein OsVSR2 and is translo-cated intracellularly from the trans-Golgi network to pre-vacuolar compartments and then to the vacuole.In this trafficking process,OsCS1 actively transports Cd into the endomembrane system and sequesters it in the vacuoles.There are natural variations in the promoter of OsCS1 between the indica and japonica rice subspecies.Duplication of a G-box-like motif in the promoter region of the superior allele of OsCS1 from indica rice enhances the binding of the transcription factor OsIRO2 to the OsCS1 promoter,thereby pro-moting OsCS1 expression.Introgression of this allele into commercial rice varieties could significantly lower grain-Cd levels compared to the inferior allele present in japonica rice.Collectively,our findings offer new insights into the genetic control of leaf-to-grain Cd translocation and provide a novel gene and its su-perior allele for the genetic improvement of low-Cd variety in rice.

riceOsCS1/OsMTP11grain CdTGN-PVC-vacuole transportallelic variation

Peng Liu、Liang Sun、Yu Zhang、Yongjun Tan、Yuxing Zhu、Can Peng、Jiurong Wang、Huili Yan、Donghai Mao、Guohua Liang、Gang Liang、Xiaoxiang Li、Yuntao Liang、Feng Wang、Zhenyan He、Wenbang Tang、Daoyou Huang、Caiyan Chen

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Key Laboratory of Agro-Ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China

University of Chinese Academy of Sciences,Beijing 100049,China

College of Agronomy,Hunan Agricultural University,Changsha 410128,China

Key Laboratory of Plant Resources,Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China

Jiangsu Key Laboratory of Crop Genetics and Physiology,Yangzhou University,Yangzhou 225009,China

CAS Key Laboratory of Tropical Plant Resources and Sustainable Use,Xishuangbanna Tropical Botanical Garden,Chinese Academy of Sciences,Kunming,Yunnan 650223,China

Hunan Rice Research Institute,Hunan Academy of Agricultural Science,Changsha 410125,China

Guangxi Key Laboratory of Rice Genetics and Breeding,Rice Research Institute,Guangxi Academy of Agricultural Sciences,Nanning 530007,China

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2024

10.1016/j.molp.2024.09.012

分子植物(英文版)
中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD
影响因子：0.659
ISSN：1674-2052
年,卷(期)：2024.17(11)