A TT1-SCE1 module integrates ubiquitination and SUMOylation to regulate heat tolerance in rice

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外文摘要：Heat stress poses a significant threat to grain yield.As an α2 subunit of the 26S proteasome,TT1 has been shown to act as a critical regulator of rice heat tolerance.However,the heat tolerance mechanisms medi-ated by TT1 remain elusive.In this study,we unveiled that small ubiquitin-like modifier(SUMO)-conjugating enzyme 1(SCE1),which interacts with TT1 and acts as a downstream component of TT1,is engaged in TT1-mediated 26S proteasome degradation.We showed that SCE1 functions as a negative regulator of heat tolerance in rice,which is associated with its ubiquitination modification.Furthermore,we observed that small heat-shock proteins(sHSPs)such as Hsp24.1 and Hsp40 can undergo SUMOylation mediated by SCE1,leading to increased accumulation of sHSPs in the absence of SCE1.Reducing protein levels of SCE1 significantly enhanced grain yield under high-temperature stress by improving seed-setting rate and rice grain filling capacity.Taken together,these results uncover the critical role of SCE1 in the TT1-mediated heat tolerance pathway by regulating the abundance of sHSPs and SUMOylation,and ultimately modulating rice heat tolerance.These findings underscore the great potential of the TT1-SCE1 module in improving the heat tolerance of crops.

外文关键词：

riceheat toleranceubiquitinationSUMOylationheat-shock protein

作者：

Hong-Xiao Yu、Ying-Jie Cao、Yi-Bing Yang、Jun-Xiang Shan、Wang-Wei Ye、Nai-Qian Dong、Yi Kan、Huai-Yu Zhao、Zi-Qi Lu、Shuang-Qin Guo、Jie-Jie Lei、Ben Liao、Hong-Xuan Lin

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作者单位：

National Key Laboratory of Plant Molecular Genetics,CAS Center for Excellence in Molecular Plant Sciences,Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,China

University of the Chinese Academy of Sciences,Beijing 100049,China

Joint Center for Single Cell Biology,School of Agriculture and Biology,Shanghai Jiao Tong University,Shanghai 200240,China

Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510642,China

School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China

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出版年：

2024

DOI：

10.1016/j.molp.2024.11.007

分子植物(英文版)

中科院上海生命科学研究院植物生理生态所　中国植物生理学会

分子植物(英文版)

CSTPCD

影响因子：0.659

ISSN：1674-2052

年,卷(期)：2024.17(12)