RAPD Analysis of Classification and Genetic Relationship among Northern Japonica Rice
In this research, we have studied the genetic relationships and classifications of tested 18 northern japonica rice based on the RAPD data though it is rarely reported on classification and genetic relationship of japonica rice by using RAPD technique in China. The results showed that 104 polymorphic bands were scored from total of 146 amplified bands, it was 71.23% polymorphic percentage and 6.95 average allele number of each polymorphic primer that should display a relatively high polymorphism revealed by RAPD-PCR using 21 random primers selected from 80 primers. We totally found out 15 primers with single-marked band or specific fragment missing, which can be used to distinguish 10 tested germplasms. And we also conducted the cluster analysis for the tested materials by using the SPSS statistic software (SPSS 11.5). The statistic results demonstrated that 18 tested varieties could be classified into three groups significantly, eight of them were directly going to Group I well according with the results of pedigree analysis, as well as the tested germplasms in group II and group HI had well relative relationships, respectively, which was basically consistent with the results of pedigree analysis. In this study we have conclusions that there are obviously differences on analyzing classification and relationship by using RAPD technique and pedigree analysis, and RAPD technique might be feasible for the research to detect the genetic diversities in northern japonica rice. Thus, we suggested that it would be best way by combining the molecular technique and pedigree analysis in practices, which will help us to reduce the blindness of parent selection and to increase the predictability in breeding program.
NETL
NSTL
万方数据
维普
