转IPT基因油菜提高抗蚜虫能力
IPT Gene Expression Increased the Aphid-resistant of Transgenic Plant in Oilseed
李海林 1赵德刚2
作者信息
- 1. 贵州大学生命科学学院,贵阳,550025;贵州大学,贵州省农业生物工程重点实验室,贵阳,550025
- 2. 贵州大学生命科学学院,贵阳,550025;贵州大学,贵州省农业生物工程重点实验室,贵阳,550025;贵州大学,教育部绿色农药与农业生物工程重点实验室,贵阳,550025
- 折叠
摘要
本研究利用农杆菌介导IPT(isopentenyl-transferases,异戊烯基转移酶)基因转化喀斯特特有甘蓝型油菜自交品种S65,在无激素的筛选培养基中获得再生植株14株,PCR检测12株外源基因整合入油菜基因组中.对其中5株转基因植株及5株野生型植株人工接种蚜虫试验,研究超量表达IPT基因油菜对蚜虫的抗性.结果表明:转IPT,基因植株表现高抗蚜虫,而野生型植株表现高感蚜虫,极易受到蚜虫侵害.人工接虫24d时,T1、T2和T3转基因植株较野生型对蚜虫的抑制率均为100%,T4和T5抑制率分别为98.59%和99.01%.表明转IPT基因油菜显著提高油菜具有的抗蚜能力,为培育抗蚜虫转基因油菜提供新品种.
Abstract
In this study, Karst unique inbred strains oilseed (Brassica napus L.) S65 was transformed by Agrobacteriurn-mediated method with the plasmids DNA containing IPT gene, 14 regenerated plants were obtained from hormone-free selection medium, and IPT gene was integrated into the genomes of 12 plants by PCR identification. The aphids-resistant of over-expression IPT gene oilseed were studied by the artificial inoculation aphids on. The result showed that transgenic oilseed at aphid-resistant was more stronger than wild-type plants.The aphids were inhibited on transgenic plants T1, T2 and T3 with 100％, T4 and T5 with 98.59％ and 99.01％respectively. The research results suggested that IPT gene expression increased the aphid-resistant of transgenic oilseed. It was provided new varieties for the cultivation of aphid-resistant transgenic oilseed.
关键词
IPT基因/转基因油菜/蚜虫/人工接虫Key words
IPT gene/Transgenic oilseed/Aphid, Artificial inoculation引用本文复制引用
基金项目
国际科技部国际合作项目(2007DFA31260)
科技部国家科技支撑计划项目(2007BAD59B06)
国家转基因新品种培育重大专项子项目(2008ZX08010-003)
出版年
2011
NETL
NSTL
维普
万方数据