The Approach for Screening Offspring of Transgenic TSVPI Maize by Herbicide Chlorsulfuron
This study optimized the procedures for screening transgenic offspring of maize by using herbicide. As the herbicide screening marker gene, ALS gene has the advantages of efficiency, speed, simpleness & convenience and so on. Utilizing the spraying method with 60 mg/L Chlorsulfuron can filter a great quantity of non-transgenic plants together with lines and some with low expression of ALS gene. This method can be used to assist screening transgenic plants offspring, improve the speed of screening positive plants, and accelerate becoming homozygosis lines and breeding transgenic maize by back-crossing.
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万方数据
维普
