The evolution of 62 wheat varieties cultivated widely in Shandong province since 1950 was evaluated by 24 pairs of SSR markers. The results revealed that all 24 pairs of SSR markers were highly polymorphic, and 100 alleles were amplified with the average of 4.167 per marker. The mean polymorphism information content (PIC) is 0.527 with a range from 0.200 to 0.723. The genetic diversity indexesrange from 0.225 to 0.761 with the average of 0.582. There were significant differences in site polymorphism among these three genomes, and the average richness of allelic variation in each genome is D>B>A. The genetic diversity index and the polymorphism information content index of genome B are similar to that of genome D, but higher obviously than that of genome A. The average genetic richness, genetic diversity index and genetic distance in 1950s are all higher, and each of them is 3.042,0.531 and 0.596 respectively. Then they decreased slowly untilthey rebounded in 1980s and reached the highest of 3.250, 0.560 and 0.616 respectively. After that they decreased sharply again. The genetic similarity coefficients of 62 wheat cultivars vary from 0.580 to 0.940, and the mean is 0.723. All wheat cultivars could cluster into 7 groups at 0.719 with the method of UPGMA. The clustering result is mostly consistent with the main parental lines and* their derivatives for wheat breeding on different period in Shandong province. It shows that the wheat breeding in Shandong province, as well as throughout China, has been based on core parental lines since 1950. Because of the creation and application of the endemic germplasm, genetic diversity of wheat cultivars released in Shandong province had even been the highest in all the wheat regions of China, especially in 1980s. But it declined sharply after 1980s. Therefore more effort needs to be made to broaden the wheat breeding base and conserve wheat germplasm.