Genetic Analysis and Molecular Mapping of a Thermo-sensitive Chlorosis Mutant in Rice
A thermo-sensitive chlorosis mutant, tcm12, derived from a progeny of a japonica rice variety, Jiahua No.1, treated by 60Co γ-radiation, displayed cholosis in the seedling of the 2nd and 3rd leaf stages under the low temperature below at 20℃, then turned normal green in the beginning of the 4th leaf growing Whereas the mutant can grow normally as the wild type variety, Jiahua No.1, at above 24℃, indicating that the trait of of chlorosis should be the thermo-sensitive. The transmission electron microscope assay showed that the chloroplast in the mutant was undeveloped under the temperature at 20℃below, but was well-developed under the condition of at 32℃. Then genetic analysis revealed that mutant trait was controlled by a single recessive nuclear gene (tcm12). Using SSR and InDel markers and 76 mutant-type F2 individuals from the population derived from the cross of Guangzhan63S/tcm12, tcm12 was roughly located between RM519 and ID22406 on the long arm of chromosome 12. Furthermore, 157 mutant-type F2 individuals from another population derived from the cross of 9311/tcm12 in view of better polymorphic markers on the target region, tcm12 was finally narrowed to in the range of 237 kb in length between the molecular markers of ID21199 and ID21436. The results obtained from the study may lay the basis for further cloning and functional analysis of this gene in rice.
NETL
NSTL
万方数据
维普
