In this study, AhCMO gene was introduced into maize inbred“Zheng58”by pollen-mediated transfor-mation approach assisted with ultrasonication. T2 transgenic seeds were obtained through antibiotic screening, PCR detection and self-pollination of T1 generation plants. Five T2 generation seeds were grown in pots to evaluate their salt tolerance. The results indicated that 250 mmol/L NaCl was the appropriate concentration for evaluating salt tolerant transgenic lines. Analysis on physiological and biochemistry indices indicated that the activities of superoxide dismutase (SOD), peroxidase (POD) and chlorophyll content of transgenic lines be higher than non-transgenic control plants, their malonaldehyde (MDA) content be lower than non-transgenic ones under the stress of 250 mmol/L NaCl. In transgenic lines, SOD activity was increased by from 2% to 208%; POD activity was increased by from 22% to 65%; chlorophyll content was increased by from 8% to 61% and MDA content was decreased by from 3%to 93%. In conclusion, the introduction of CMO gene improved salt tolerance of the maize plants; Based on evaluation result, two of the five transgenic lines had salt-tolerance levels scored three grades higher than the control.
MaizeAtriplex hortenis choline monooxygenase gene (AhCMO)Genetic transformationSalt physio-logy and biochemistry indices
NETL
NSTL
万方数据
维普
