Identification and Bioinformatics Analysis of Chalcone Synthase Genes in Tomato
Flavonoids are a kind of important secondary metabolites in plants. Usually, it was found in fruits, vegetables, beans, tea and many other plants as combination (flavonoid glycosides) or free states (flavonoid glyco-sides) form. It has important role in regulating plant growth and development. Chalcone synthase, the first key synthase during the process of flavonoids synthesis, plays an important role in plant growth and development. Based on the whole tomato genome sequence, we investigated gene members of the chalcone synthase family with genome database and bioinformatics analysis. We identified 8 chalcone synthase genes with protein sequence length varying from 160 to 438. Sequence identity analysis was further performed. The lowest value was observed for SlCHS02 and SlCHS06 (33.7%) and the highest value was observed for SlCHS04 and SlCHS07 (92.0%). The result showed high degrees of variation among these genes. Gene structure analysis showed few introns in these genes. Sequence alignment found that these genes were high conserved. Moreover, these SlCHS genes distribute in chromosome 1, 5, 6, 9 and 12 in tomato. The study not only will help us understand the origin and evolution of these gene families, but also lay the foundation for analyzing functions of the gene family members.
NETL
NSTL
万方数据
维普
