花生基因工程研究进展
Advances in Genetic Engineering of Peanut (Arachis hypogaea L.)
黄冰艳 1张新友 1苗利娟 1齐飞艳 1石磊 1董文召 1汤丰收1
作者信息
- 1. 河南省农业科学院经济作物研究所,农业部黄淮海油料作物重点实验室,河南省油料作物遗传改良重点实验室,郑州,450002
- 折叠
摘要
本文归纳了近十几年来国内外花生遗传转化方法,分析了不同方法的诱导效率和转化效果,概括了花生转基因研究进展并对其应用前景进行了展望.常用的花生转基因方法主要有3种,一是基因枪法转化胚性组织,二是农杆菌介导转化子叶(节),三是农杆菌介导转化茎尖(非组培).花生基因工程转化的目的基因主要包括提高对病毒、真菌、干旱、除草剂等抗性的相关基因,在降低花生过敏原、提高油酸含量等品质改良和生物反应器生产免疫产品等方面也开展了探索性研究.花生体细胞胚诱导及再生和农杆菌介导的遗传转化技术日渐成熟,但花生的遗传转化体系仍存在转化效率低、受体基因型范围窄、后代遗传不稳定等问题,建立稳定、高效的转化平台是实现花生基因工程潜在价值的基础.
Abstract
The techniques of genetic transformation being applied on peanut in the last decade were reviewed.The progress on genetic engineering of peanut was summarized and its prospect was discussed by comparing the transformation efficiency and effects of transformed genes.There were mainly three systems for genetic engineering of peanut including particle bombardment of embryonic callus,Agrobacterium mediated transformation of cotyledon section,and A grobacterium mediated transformation of apical meristems (tissue culture-independent).The targeted traits included enhancing plant resistance to virus and fungi infection and tolerance to drought and herbicide,and few were reported on reducing peanut allergens,improving oleic acid content and producing vaccines.Even though embryogenesis and Agrobacterium mediated transformation were available for some genotypes,the low efficiency of transformation,few suitable genotypes and unstable heredity of transformed genes were to be improved further to establish reliable platform of peanut genetic engineering.
关键词
花生/组织培养/遗传转化/基因工程Key words
Peanut (groundnut, Arachis hypogaea L.)/Tissue culture/Genetic transformation/Genetic engineering引用本文复制引用
基金项目
国家花生产业技术体系(CARS-14)
河南省重大科技专项(111100110200)
河南省农业科学院科研发展专项资金项目(201218305)
出版年
2015
NETL
NSTL
维普
万方数据