Transferability of Soybean Gemonic-SSR and EST-SSR Markers in Astragalus
Transferability of molecular markers has the vital significance to reduce their development cost, improve their development efficiency and promote molecular genetics research of some species which genetic research is relatively weaker. For the development of SSR molecular markers in Astragalus, a popular Chinese medicinal plant, 66 pairs of genomic-SSR primers and 43 pairs of EST-SSRs primers designed from soybean were used to amplify the genomic DNA of Astragalus. Among 109 pairs of primers, 23 pairs of primers with distinct bands were selected to analyze genetic information of 6 Astragalus from different sources. The results showed that the transferability rate of soybean genomic-SSR and EST-SSR in Astragalus was 31.82%and 76.74%, respectively. The percentage of polymorphism of loci of effective genomic SSR primers was 18.18%, whereas EST-SSRs reached 53.49%of polymorphism in Astragalus. The EST-SSRs of soybean had higher transferability to Astragalus than that of genomic-SSRs. The soybean SSR markers can be used for population genetic analysis and diversity analysis in Astragalus. The polymorphic SSR markers identified in this study are useful for further research of genetic diversity in Astragalus.
NETL
NSTL
万方数据
维普
