Identification and Bioinformatics Analysis of the PIN Gene Family in Jatropha curcas
PIN gene family is a kind of important carrier elements regulating auxin polar transport. In this paper, the nine PIN genes (JcPIN) were identified in the genome data of Jatropha curcas using Arabidopsis thaliana PIN protein family as reference sequences. And the analysis of phylogenetic relationships, motif composition, expression character, and comparison of codon usage bias were performed in JcPIN genes. The results showed that the most of JcPIN genes belongs to the stable proteins, which composed of basic amino acids. Nine JcPIN genes are non-secreted proteins located on the cell membrane with N terminal conserved domains. Secondary structure of PIN proteins is similar between Jatropha curcas and A rabidopsis thaliana. The phylogenetic analysis displayed that Jatr-opha curcas PIN genes have the closest relationship with Populus trichocarpa PIN genes. Expression analysis found four high credibility genes PIN genes of Jatropha curcas. Analysis of codon usage bias identified thirteen high-frequency codons in JcPIN genes, and revealed that optimized codon of JcPIN genes for heterologous expression.The results here would provide an important foundation for future functional studies of Jc PIN gene family, and can also be useful for other gene family of Jatropha curcas and other plant gene family with a large amount of data.