Molecular Cloning and Expression Analysis of CIPK 23 Gene from Sugarcane under Low Potassium, Drought and Salt Stress
In this study, A CBL-interacting protein kinase gene named SsCIPK23 (GenBank accession number:KM981737) was cloned by reverse transcription-polymerase chain reaction (RT-PCR) from root of sugarcane und-er low potassium stress. The full length of SsCIPK23 gene was 1 749 bp, containing a 1 350 bp complete open reading frame, which encoded 449 amino acids. The SsCIPK23 contained a protein kinase and a NAF domain which are the typical domains of CIPK family. The phylogenetic analysis of SsCIPK23 with CIPK family in various plants revealed that SsCIPK23 was closely related with CIPK23 of various plants and the CIPK23 genes in various plants were highly conservative. The result of qPCR indicated that the expression of SsCIPK23 was changed with low potassium, drought and salt stresses. Under low potassium stress, the expression of SsCIPK23 was up-regulated at 24 h under, and the relative expression increased with the stress time;under drought and salt stress, the highest relative express of SsCIPK23 appeared at 48 h and then relative express decreased at 92 h. The results revealed that the SsCIPK23 might take part in responses to various stress. It builds a great base for researching functional of SsCIPK23.
NETL
NSTL
万方数据
