首页|马尾松PmTIR 1基因的克隆及编码蛋白质的结构预测

马尾松PmTIR 1基因的克隆及编码蛋白质的结构预测

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TIR1(transport inhibitor response 1)基因在生长素信号转导途径中发挥重要作用。本研究采用PCR技术和RACE技术，利用Primer Premier5进行引物设计，从马尾松中克隆出TIR1基因的全长cDNA序列，命名为PmTIR 1。此cDNA全长2446 bp，包括1725 bp完整的ORF，能够编码含有574个氨基酸的蛋白，此蛋白相对分子质量为64240.3 u，等电点为6.76。利用ClustalX、ANTHEPROT和DNAMAN等软件分析PmTIR 1序列及其编码的氨基酸序列，结果表明：马尾松PmTIR1蛋白氨基酸序列与火距松TIR1蛋白同源性高达99％，PmTIR1蛋白含有F-box结构域和多个LRR结构域；PmTIR1蛋白的F-box结构域可能与SCFTIR1中SKP1结合，它符合F-box家族的特点。利用实时定量技术得知PmTIR 1在马尾松嫩根、嫩茎、嫩叶中都有表达，在嫩叶中表达量最高、嫩根中次之、嫩茎中最低。本研究可为生长素调控马尾松生长发育相关机理提供理论依据。
Cloning and Coding Protein Structure Prediction of TIR 1 Gene from Pinus mas s oniana
TIR1 (transport inhibitor response 1) plays an important role in auxin signal transduction pathway. In this study, we used primer 5 to design primers, through PCR and RACE technology, the TIR1 gene from Pinus massoniana named PmTIR1 was cloned. PmTIR1, having 2 446 bp in full length, contains an opening frame of 1 725 bp that encodes a protein of 574 amino acids with predicted relative molecular mass of 64 240.3 u and PI of 6.76. Its nucleotide sequence and the encoded amino acid sequence were analyzed with softwares of ClustalX, DNAMAN and ANTHEPROT, the results showed that:the homology of PmTIR1 protein from Pinus massoniana and TIR1 protein from Pinus tae da was as high as 99%, PmTIR 1 contained the F-box protein domains and multiple LRR domain;F-box protein domains in PmTIR 1 may combine with SKP1 in SCFTIR1, it was consistent with the characteristics of F-box family. Using real-time quantitative techniques, we identified the amount of PmTIR 1 expression, which were the highest in leaf and the lowest in stem from P. massoniana. The study can help to discover how the auxin regulating growth and development of Pinus massoniana.

Pinus massonianaAuxinTIR1 (transport inhibitor response 1)Sequence analysisStructure predi-ction

苏江、郭天玮、季孔庶

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南京林业大学南方现代林业协同创新中心，南京，210037

马尾松 生长素 TIR1 (transport inhibitor response 1) 序列分析 结构预测

国家“十二五”科技支撑项目林业公益性行业科研专项经费江苏高校优势学科建设工程项目南方现代林业协同创新项目共同资助

2012BAD01B02201104010PAPD

2015

10.13271/j.mpb.013.001355

分子植物育种
海南省生物工程协会

分子植物育种

CSTPCDCSCD北大核心
影响因子：0.765
ISSN：1672-416X
年,卷(期)：2015.(6)
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